잠시만 기다려 주세요. 로딩중입니다.

Recombinant AtNF-YA, a subunit of theArabidopsis CCAAT-binding transcription factor, forms a protein-DNA complex with mammalian NF-YB and NF-YC

한국식물학회지 2006년 49권 4호 p.281 ~ 285
박수민 ( Park Soo-Min ) - University of California Division of Biological Sciences

Abstract


The evolutionary conserved CCAAT binding protein NF-Y is a common regulatory DNA binding protein consisting of three distinct subunits. Unlike yeast and mammals, in which only a single copy of each subunit is encoded,Arabidopsis encodes a multi-gene family for each subunit in its genome. Compared with the NF-Y of mammals or yeast, very little is known about plant NF-Y homologs. HereArabidopsis NF-YA subunits were isolated to determine whether they could form a hete-rotrimeric NF-Y complex with mammalian NF-YB and NF-YC. This resultant chimeric NF-Y complex had DNA binding ability to the same CCAAT sequences as those of the other life systems. Therefore, it is possible that plant NF-Y homologs might have biochemical characteristics similar to mammalian NF-Y, thereby suggesting its functional conservation among organisms.

키워드

Arabidopsis genome; electrophoretic mobility shift assay; Nuclear Factor Y; yeast three-hybrid assay
원문 및 링크아웃 정보
 
등재저널 정보