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Analysis of gene expression upon DNA damage inArabidopsis

한국식물학회지 2006년 49권 4호 p.298 ~ 302
김윤성,
소속 상세정보
김윤성 ( Kim Youn-Sung ) - Seoul National University Department of Chemistry

Abstract


To understand their responses to DNA damage,Arabidopsis plants were exposed to methyl methanesulfonate (MMS). Such treatment inhibited growth and decreased the leaf chlorophyll content. A concomitant change in the expression of cell cyclerelated genes also occurred.CYCB1 expression was slightly increased but that ofCYCD1 declined. To identify the genetic elements in these responses, genome-wide transcription profiling ofArabidopsis was performed following the MMS treatment, using a cDNA microarray. Expression was altered by more than two-fold for 3666 genes ? i.e., 1657 genes showed an increase and 2009 genes had a decrease in transcripts. Five DNA repair-related genes were slightly enhanced, while several disease resistance-related and glutathione transferase genes were strongly up-regulated. Interestingly, 27 ubiquitin-related genes were also altered by more than two-fold, suggesting that protein degradation may have been involved in those damage responses. These microarray results were validated by RT-PCR.

키워드

Arabidopsis; DNA damage; methyl methanesulfonate; microarray

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