Somatic embryogenesis and plantlet formation from a rare and endangered tree species,Oplopanax elatus
문흥규, Kim Ji-Ah, 박소영, Kim Yong-Wook, 강호덕,
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문흥규 ( Moon Heung-Kyu ) - Korea Forest Research Institute Biotechnology Division
( Kim Ji-Ah ) - Korea Forest Research Institute Biotechnology Division
박소영 ( Park So-Young ) - Korea Forest Research Institute Biotechnology Division
( Kim Yong-Wook ) - Korea Forest Research Institute Biotechnology Division
강호덕 ( Kang Ho-Duck ) - Dongguk University Department of Forestry Resources
Abstract
We tested the possibility of plantlet formation via somatic embryogenesis with leaf segments and mature zygotic embryos from a rare and endangered tree species,Oplopanax elatus. To induce calli, expiants were cultured under darkness in a solid MS medium containing 3% sucrose, 1g L-1 glutamine, and 0.3% gelrite. Treatment supplements included 2,4-D alone or in combination with thidiazuron. Generally, callus induction and growth were good from leaf expiants, whereas embryogenic calli could be induced only from zygotic embryos. These embryogenic calli were white or pale yellow and very friable. ABA and activated charcoal appeared to be important factors when inducing somatic embryos, with optimum levels being 0.1 mg L-1 and 0.02%, respectively. Many somatic embryos showed abnormalities during their development on the germination medium, but 35% could be converted if placed on a medium containing gibberellic acid (GA3). The germinating embryos sometimes formed secondary embryos at the lower portion of the hypocotyls. Normal or converted plantlets were acclimatized in an artificial soil mixture; their survival was about 60% after two months. This culturing system provides a feasible approach for regenerating plants, via somatic embryogenesis, from mature zygotic embryos.
키워드
micropropagation; Oplopanax elatus; plantlet formation; somatic embryogenesis; zygotic embryos
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