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Characterization of an apple anthocyanidin synthase gene in transgenic tobacco plants

한국식물학회지 2006년 49권 4호 p.326 ~ 326
 ( Kim Sung-Hyun ) - Sogang University Department of Life Science

이재린 ( Lee Jae-Rin ) - Sogang University Department of Life Science
김성룡 ( Kim Seong-Ryong ) - Sogang University Department of Life Science

Abstract


Anthocyanin is the major color pigment in plants. The apple anthocyanidin synthase gene(ANS) manifests fruit skin-preferential expression (Kim et al., 2003). To understand the regulatory mechanism for such expression, we isolated and analyzed an appleANS genomic clone. Sequence analysis of ca. 1.4-kb of theANS promoter region predicted several cis-elements for MYB, light responsive GT-1, and the ABA Responsive Element (ABRE). Transgenic tobacco plants carrying a chimeric fusion between theANS promoter and the β-glucuronidase gene(GUS) showed that GUS was expressed in the receptacles and immature seeds as well as in the floral buds, but not in the vegetative organs.

키워드

anthocyanidin synthase gene; anthocyanin; apple; GUS; promoter
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