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Optimization of direct somatic embryogenesis from mature zygotic embryos ofPanax ginseng C. A. Meyer

한국식물학회지 2006년 49권 5호 p.348 ~ 348
김옥태 ( Kim Ok-Tae ) - RDA National Institute of Crop Science

김태수 ( Kim Tae-Soo ) - RDA National Institute of Crop Science
 ( In Dong-Soo ) - RDA National Institute of Crop Science
방경환 ( Bang Kyong-Hwan ) - RDA National Institute of Crop Science
김영창 ( Kim Young-Chang ) - RDA National Institute of Crop Science
최용의 ( Choi Yong-Eui ) - Kangwon National University College of Forest Sciences Division of Forest Resources
차선우 ( Cha Seon-Woo ) - RDA National Institute of Crop Science
성낙술 ( Seong Nak-Sul ) - RDA National Institute of Crop Science

Abstract


Culture conditions were optimized for somatic embryogenesis ofPanax ginseng. The highest frequency of embryo formation was obtained when tissues were excised from the middle region of the cotyledon segments of zygotic embryos. Only treatment with light could stimulate the formation of single-type somatic embryos, whereas multiple-type somatic embryos and calli were observed under dark conditions. The highest production of somatic embryos was found with an NH4 +:NO3 ratio of 21:39. Among the tested media (MS, B5, and SH), maximum formation of somatic embryos was obtained when cotyledon expiants were cultured on an 1% agar MS medium supplemented with 5% sucrose. Regenerated ginseng plantlets were transferred to an autoclaved soil mixture in the greenhouse. These transformants showed no detectable variations in their morphology or growth characteristics compared with the donor plant.

키워드

agar; macro salts; Panax ginseng; somatic embryogenesis; sucrose
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