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Determination of self-locompatibility genotypes of Korean apple cultivars based on S-RNase PCR

한국식물학회지 2006년 49권 6호 p.448 ~ 448
김회택 ( Kim Hoy-Taek ) - Chiba University Faculty of Horticulture

 ( Hattori Gen ) - Tokyo University of Agriculture and Technology United Graduate School of Agricultural Science
 ( Hirata Yutaka ) - Tokyo University of Agriculture and Technology United Graduate School of Agricultural Science
 ( Kim Dae-Ill ) - RDA National Horticultural Research Institute Fruit Tree Breeding Division
 ( Hwang Jeong-Hwan ) - RDA National Horticultural Research Institute Fruit Tree Breeding Division
 ( Shin Yong-Uk ) - RDA National Horticultural Research Institute Fruit Tree Breeding Division
 ( Nou Ill-Sup ) - Suncnon National University Faculty of Plant Science and Production

Abstract


To prevent self-fertilization, apple has a gametophytic self-incompatibility mechanism, part of a widespread intraspecific system, that is controlled by a multi-allelic locus. This attribute has been exploited in breeding programs for new cultivars. Likewise, many apple orchards depend on artificial pollination. Therefore, molecular analysis and early identification of the self-incompatibility (S) genotype could greatly improve breeding schemes and pollen donors selection. Here, we PCR-amplified the S-RNase PCR fragments from a total of 14 cultivars and parents, using new primers (ASPF3+ASPR3) common to 23 S-alleles in apple. The S-genotypes were determined for the following: ‘Hongro’ (S1S3), ‘Gamhong’ (S1S9), ‘Saenara’ (S1S3), ‘Chukwang’ (S3S9), ‘Hwahong’ (S3S9), ‘Seokwang’ (S3S3), ‘Hwarang’ (S1S9), ‘Sunhong’ (S3S9), ‘S.E.B.’ (S1S19), ‘S.G.D.’ (S2S3), and ‘Mollie’s Delicious’ (S3S7). We also confirmed the characteristics of the S-genotypes for eight Korean apple cultivars by PCR-Southern blot analysis, using seven S-RNases as probes.

키워드

apple; gametophytic self-incompatibility; PCR; S-genotype; S-RNase
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