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A La-Related Protein LaRP6a Delays Flowering of Arabidopsis thaliana by Upregulating FLC Transcript Levels

한국식물학회지 2020년 63권 5호 p.369 ~ 378
Park Su-Jung, Lee Hwa-Jung, Lee Kwan-Uk, Kang Hun-Seung,
소속 상세정보
 ( Park Su-Jung ) - Chonnam National University College of Agriculture and Life Sciences Department of Applied Biology
 ( Lee Hwa-Jung ) - Chonnam National University College of Agriculture and Life Sciences Department of Applied Biology
 ( Lee Kwan-Uk ) - Chonnam National University College of Agriculture and Life Sciences Department of Applied Biology
 ( Kang Hun-Seung ) - Chonnam National University College of Agriculture and Life Sciences Department of Applied Biology

Abstract


La-related proteins (LaRPs) are RNA-binding proteins (RBPs) that contain a La-type helix-turn-helix (HTH) domain and are found in all eukaryotes, including plants, animals, and yeasts. Although the functions of a few LaRPs have been determined during plant growth and development, the roles of most LaRPs in plants remain unknown. Here, the function of an Arabidopsis thaliana LaRP6a (At5g46250) harboring a La-type HTH domain and an RNA-recognition motif was determined in the growth and development of Arabidopsis. Confocal analysis of subcellular localization of the LaRP6a?GFP fusion protein revealed that LaRP6a protein is localized to both the nucleus and plasma membrane. Overexpression of LaRP6a resulted in delayed flowering and increased plant height and seed yield. The transcript level of the floral repressor FLC was markedly increased in LaRP6a-overexpressing transgenic plants. LaRP6a protein bound to the 3?UTR of the FLC transcript and possessed RNA chaperone activity. Collectively, these results suggest that LaRP6a plays a role in the control of flowering time by regulating FLC transcript levels.

키워드

Arabidopsis; Flowering; La-related protein; La-type HTH; RNA-binding protein

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