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NH4+ Suppresses NO3?-Dependent Lateral Root Growth and Alters Gene Expression and Gravity Response in OsAMT1 RNAi Mutants of Rice (Oryza sativa)

한국식물학회지 2020년 63권 5호 p.391 ~ 407
Kumar Vikranth, 김성훈, Priatama Ryza A., 정진희, Adnan Moch Rosyadi, Saputra Bernet Agung, 김철민, 제병일, 박순주, 정기홍, 김경민, Xuan Yuan Hu, 한창덕,
소속 상세정보
 ( Kumar Vikranth ) - Gyeongsang National University Division of Applied Life Science
김성훈 ( Kim Sung-Hoon ) - Gyeongsang National University Division of Applied Life Science
 ( Priatama Ryza A. ) - National Fusion Research Institute Plasma Technology Research Center
정진희 ( Jeong Jin-Hee ) - Gyeongsang National University Division of Applied Life Science
 ( Adnan Moch Rosyadi ) - Gyeongsang National University Division of Applied Life Science
 ( Saputra Bernet Agung ) - Gyeongsang National University Division of Applied Life Science
김철민 ( Kim Chul-Min ) - Wonkwang University Division of Horticulture Industry
제병일 ( Je Byoung-Il ) - Pusan National University College of Natural Resources and Life Science Department of Horticultural Bioscience
박순주 ( Park Soon-Ju ) - Wonkwang University Department of Biological Science
정기홍 ( Jung Ki-Hong ) - Kyung Hee University Graduate School of Biotechnology
김경민 ( Kim Kyung-Min ) - Kyungpook National University College of Agriculture and Life Science School of Applied Biosciences
 ( Xuan Yuan Hu ) - Shenyang Agricultural University College of Plant Protection
한창덕 ( Han Chang-Deok ) - Gyeongsang National University Division of Applied Life Science

Abstract


The AMT1 family comprises major ammonium transporters in rice roots. In this study, we utilized AMT1 RNAi mutants (amt1) to explore how AMT1 affects NH4+- and NO3?-mediated morphological development and NH4+-responsive gene expression in roots. In the presence of NH4+, amt1 showed inhibition of NO3?- dependent lateral root development. The inhibitory action of NH4+ on lateral root growth was independent of the NO3? concentrations supplied to amt1 roots. The results of split root assays indicated that NH4+ exerts systemic action in inhibiting NO3?-dependent lateral root development in amt1. Further study with NAA and NOA, a potent auxin flux inhibitor, suggested that perturbation of membrane dynamics might not be the primary cause of the inhibitory action of NH4+ on NO3?-mediated lateral root growth in amt1 mutants. RNA-seq analysis of NH4+-responsive genes showed that approximately half of DEGs observed in wild-type roots were not detected in the DEGs of amt1 roots. Gene ontology enrichment analysis suggested that the expression of specific functional gene groups were affected by amt1 during the early response to NH4+. Auxin-responsive gene expression and root gravity responses were altered in amt1. This study demonstrated that AMT1 affects the interactions not only between ammonium and nitrate in lateral root growth but also between auxin and NH4+ in rice roots.

키워드

Rice; AMT1; Ammonium; Lateral roots

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