잠시만 기다려 주세요. 로딩중입니다.

4년근 미성숙 인삼열매 분획물의 약리학적 특성 및 치매 마우스 모델에서 인지능 개선효과

Pharmacological Properties of the Four-year Unripe Ginseng Berry and the Enhancing Effect on Learning and Memory in Aβ42-induced Alzheimer Mouse Model

약학회지 2020년 64권 2호 p.109 ~ 123
안정원 ( Ahn Jeong-Won ) - Gangneung-Wonju National University College of Life Science

장수길 ( Jang Su-Kil ) - Novelgenmed R&D Center
김현수 ( Kim Hyun-Soo ) - Gangneung-Wonju National University College of Life Science
조보람 ( Jo Bo-Ram ) - Gangneung-Wonju National University College of Life Science
성은아 ( Sung Eun-Ah ) - Gangneung-Wonju National University College of Life Science
김승태 ( Kim Seung-Tae ) - Gangneung-Wonju National University College of Life Science
 ( Choi Ehn-Kyoung ) - Chungbuk National University College of Veterinary Medicine
김윤배 ( Kim Yun-Bae ) - Chungbuk National University College of Veterinary Medicine
박희용 ( Park Hee-Yong ) - Chung-Ang University College of Pharmacy
주성수 ( Joo Seong-Soo ) - Gangneung-Wonju National University College of Life Science

Abstract


In the present study, we aimed to determine whether the processed 4-year ginseng berry extract (SGF) had pharmacological properties with improving learning and memory in an Aβ42-induced Alzheimer’s mouse model. Passive avoidance test (PAT) and Morris water-maze test (MWMT) were performed after the administration with SGF, and assays (acetylcholine, ACh and Aβ) in the brain lysates were followed along with the glial fibrillary acidic protein (GFAP) detection for the brain damage. Acetylcholinesterase (AChE) activity and the gene expression (choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT) and brain-derived neurotrophic factor (BDNF)) in N2a cells was further analyzed using Ellman’s and qPCR assays, respectively. Results demonstrated that SGF contained a high amount of 3 active ginsenosides (Rg3, Rg5 and F4) and significantly improved PAT and MWMT compared to Aβ42-induced mouse model. Interestingly, AChE activity in the brain lysates was significantly reduced, whereas ACh amounts was escalated in SGF groups. In addition, ChAT, VAChT and BDNF mRNAs were significantly upregulated in the presence of a single Rg3, Rg5 and F4 as well as SGF. Taken together, these findings clearly suggest that SGF can participate in alleviating AD pathogenesis by preventing the brain damage and increasing ACh production which are primitive requirements for a therapeutic candidate of Alzheimer’s disease.

키워드

ginseng berry; passive avoidance test; Morris water-maze test; acetylcholine; choline acetyltransferase; ginsenoside
원문 및 링크아웃 정보
 
등재저널 정보