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Overexpression of Nrf2 promotes colon cancer progression via ERK and AKT signaling pathways

Annals of Surgical Treatment and Research 2020년 98권 4호 p.159 ~ 167
이윤진 ( Lee Yoon-Jin ) - Soonchunhyang University College of Medicine Molecular Cancer Research

김우일 ( Kim Woo-Il ) - Soonchunhyang University College of Medicine Molecular Cancer Research
배진호 ( Bae Jin-Ho ) - Soonchunhyang University College of Medicine Molecular Cancer Research
조문균 ( Cho Moon-Kyun ) - Soonchunhyang University College of Medicine Molecular Cancer Research
이상한 ( Lee Sang-Han ) - Soonchunhyang University College of Medicine Molecular Cancer Research
남해선 ( Nam Hae-Seon ) - Soonchunhyang University College of Medicine Molecular Cancer Research
최인호 ( Choi In-Ho ) - Soonchunhyang University College of Medicine Molecular Cancer Research
조성우 ( Cho Sung-Woo ) - Soonchunhyang University Seoul Hospital Department of Surgery

Abstract


Purpose: We investigated the expression of Nrf2 in colorectal cancer and its correlation with clinicopathological characteristics as well as mechanisms and roles of Nrf2 expression including cell signaling pathway, survival, proliferation, and migration.

Methods: Nrf2 expression was measured in 12 and 30 different colorectal cancer (CRC) tissues by western blot (WB) and immunohistochemistry (IHC), respectively. SW480 cells were used for cell proliferation and cell migration tests. The correlation between the expression of Nrf2 and clinicopathologic parameters were evaluated using the chi-square or Fisher exact test. Data are expressed as the mean ± standard deviation for 3 independent experiments. P < 0.05 was considered statistically significant.

Results: Analysis of WB demonstrated that Nrf2 proteins were increased in CRC tissues, and decreased in normal tissues. IHC staining showed that the Nrf2 expression was elevated in CRC tissues, compared to matched normal tissues. When SW480 cells were suppressed with small interfering RNA of Nrf2, cell viability was inhibited, and cell apoptosis was increased. These results were found along with suppression of the phosphorylated form of extracellular signal-regulated kinase 1/2 and AKT.

Conclusion: This study suggests that overexpression of Nrf2 may be related to carcinogenesis and progression of CRC.

키워드

Colorectal neoplasms; NF-E2-related factor 2; Reactive oxygen species
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