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Calocybe indica의 배양적 특성과 균사 배양 적합 조건 설정

Culture Characteristics and Optimal Conditions for Mycelial Growth of Calocybe indica

한국균학회지 2020년 48권 3호 p.273 ~ 284
민경진, 박혜성, 이은지, 이찬정,
소속 상세정보
민경진 ( Min Gyeong-Jin ) - RDA National Institute of Horticultural and Herbal Science Mushroom Science Division
박혜성 ( Park Hea-Sung ) - RDA National Institute of Horticultural and Herbal Science Mushroom Science Division
이은지 ( Lee Een-Ji ) - RDA National Institute of Horticultural and Herbal Science Mushroom Science Division
이찬정 ( Lee Chan-Jung ) - RDA National Institute of Horticultural and Herbal Science Mushroom Science Division

Abstract


Calocybe indica is an edible mushroom commercially cultivated in India and other tropical countries. In this study, the culture characteristics and optimal conditions of milky mushroom strains were determined. The growth temperature and pH range of milky mushrooms was extensively investigated between 15?35 ℃ and pH 3?11. For efficient cultivation, 20 types of nutrient sources were selected that consisted of one of 21 types of carbon sources, 6 organic nitrogen sources, 6 inorganic nitrogen sources, 13 amino acids, 6 organic acids and 12 inorganic salts. The impact of each of the selected nutrition sources and their concentration on growth was investigated. The optimum pH and temperature were determined to be pH 6.0 and 15 ℃, respectively. The optimum concentration of medium elements for the mycelial growth of C. indica was determined to be as follows: carbon source, 2% maltose; organic nitrogen source, 1% yeast extract; inorganic nitrogen source, 0.1% NaNO3; amino acid, 0.7% asparagine; organic acid, 0.07% acetic acid; inorganic salt, 0.07 mM MnSO4.

키워드

Calocybe indica; Culture characteristics; Milky mushroom; Optimal conditions; White summer mushroom

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