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형태적 특징 및 다좌위 염기서열 분석에 의한 산철쭉 모무늬병균 Sphaerulina azaleae 동정

Identification of Sphaerulina azaleae on Korean Azalea in Korea Based on Morphological Characteristics and Multilocus Sequence Typing

한국균학회지 2020년 48권 3호 p.329 ~ 335
최인영, 최영준, 이귀재, Ju Ho-Jong, 조성완, 신현동,
소속 상세정보
최인영 ( Choi In-Young ) - Jeonbuk National University Department of Agricultural Biology
최영준 ( Choi Young-Joon ) - Kunsan National University Department of Biology
이귀재 ( Lee Kui-Jae ) - Chonbuk National University College of Environmental and Bioresource Sciences Division of Biotechnology
 ( Ju Ho-Jong ) - Jeonbuk National University Department of Agricultural Biology
조성완 ( Cho Seong-Wan ) - Konyang University Department of Pharmaceutics & Biotechnology
신현동 ( Shin Hyeon-Dong ) - Korea University Division of Environmental Science and Ecological Engineering

Abstract


From 2008 to 2017, Korean azalea (Rhododendron yedoense f. poukhanense) showing angular, necrotic leaf spots were found in Jeju and Hongcheon, Korea. The lesions occurred frequently, detracting from the beauty of the glossy green leaves of the plant and causing premature defoliation. Therefore, to identify the fungus associated with the lesions, morphological characterization and molecular phylogenetic analysis of actin (Act), translation elongation factor 1-alpha (EF), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II encoding the second largest subunit (RPB2) of the two representative isolates were performed. The phylogenetic tree inferred from the neighbor-joining method showed the isolates clustering in the Sphaerulina azaleae group. Therefore, the fungus associated with the angular leaf spots on the Korean azalea was identified as Sphaerulina azaleae.

키워드

Angular leaf spot; Korean azalea; Rhododendron yedoense f. poukhanense; Sphaerulina azaleae

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