잠시만 기다려 주세요. 로딩중입니다.

Toxicological effects of urban particulate matter on corneal and conjunctival epithelial cells

Toxicological Research 2020년 36권 4호 p.311 ~ 318
현수왕, 송수정, Park Bong-Kyun, Lee Tae-Gu, 김찬식,
소속 상세정보
현수왕 ( Hyun Soo-Wang ) - Korea Institute of Oriental Medicine Herbal Medicine Research Division
송수정 ( Song Su-Jeong ) - Korea Institute of Oriental Medicine Herbal Medicine Research Division
 ( Park Bong-Kyun ) - Korea Institute of Oriental Medicine Clinical Medicine Division
 ( Lee Tae-Gu ) - Korea Institute of Oriental Medicine Clinical Medicine Division
김찬식 ( Kim Chan-Sik ) - Korea Institute of Oriental Medicine Clinical Medicine Division

Abstract


Exposure to urban particulate matter (UPM) is a high-risk factor for various ocular surface diseases, including dry eye syndrome. However, the effects of UPM on corneal and conjunctival epithelium damage have not been fully elucidated. In this study, we investigated the toxicological effects of UPM exposure at high concentrations by using in vitro cultures. The cell viability, mucin expression, and the secreted inflammatory mediators of corneal and conjunctival epithelial cells was observed at 24 h after exposure to UPM. The progression of cell cycle was also examined by flow cytometry at 24 h after exposure to UPM. UPM reduced cell viability in a dose-dependent manner and increased cell population in S and G2 phase. The expression of mucin-1 was attenuated by UPM exposure, but that of mucin-4 was not. UPM increased interleukin (IL)-6 release and decreased IL-8 release. The intensity of 2′,7′-dichlorofluorescein diacetate (DCF-DA) was highest at 4 h of UPM exposure. In conclusion, these results suggest that UPM causes the disruption of corneal and conjunctival epithelium by decreasing cell viability, altering cell cycle, disrupting mucin, and regulating inflammatory mediators.

키워드

Cell cycle; Cell death; Corneal/conjunctival epithelial cells; Mucin; Urban particulate mater

원문 및 링크아웃 정보

 

등재저널 정보