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Investigation of the genotoxic effects of fluoride on a bone tissue model

Toxicological Research 2020년 36권 4호 p.337 ~ 342
Volobaev V. P., Serdyukova E. S., Kalyuzhnaya E. E., Schetnikova E. A., Korotkova A. D., Naik A. A., Bach S. N., Prosekov A. Y., Larionov A. V.,
소속 상세정보
 ( Volobaev V. P. ) - Kemerovo State University Department of Genetics
 ( Serdyukova E. S. ) - Tomsk State University
 ( Kalyuzhnaya E. E. ) - Kemerovo State University Department of Genetics
 ( Schetnikova E. A. ) - Kemerovo State University Department of Genetics
 ( Korotkova A. D. ) - Kemerovo State University Department of Genetics
 ( Naik A. A. ) - Kemerovo State University Department of Genetics
 ( Bach S. N. ) - Kemerovo State University Department of Genetics
 ( Prosekov A. Y. ) - Kemerovo State University Department of Genetics
 ( Larionov A. V. ) - Kemerovo State University Department of Genetics

Abstract


Fluorides are thought to be a major cause of osteocarcinogenesis, due to their widespread industrial use, ability to accumulate in bone tissue, and genotoxic and probable carcinogenic properties. In vitro experiments investigating the genotoxic potential of fluorides in bone tissue models can provide valuable indirect information on their involvement in osteocarcinogenesis. Here, we investigated whether sodium fluoride (NaF) has the ability to induce DNA damage and chromosomal abnormalities in human osteosarcoma cells after 48 and 72 h of exposure. The cell cultures were treated with NaF in concentrations of 0, 20, 100 and 200 μg/ml. The level of DNA damage was assessed by the comet assay, and the frequency of chromosomal abnormalities by a micronucleus test. A significant increase in DNA damage indicators was noted in the samples treated with fluoride concentrations of 100 and 200 μg/ml, after 48 and 72 h of exposure. The micronucleus test revealed a dose-dependent increase in cells with micronuclei, nucleoplasmic bridges and nuclear protrusions. Increasing the concentration of NaF led to an increase in the prevalence of cytogenetic indicators after both treatment durations. This demonstrated ability of fluorine to exert genotoxic effects on bone cells indirectly indicates the possible importance of fluoride in the aetiology of osteosarcoma.

키워드

Genotoxicity; NaF; In vitro; DNA comets; Micronucleus test

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