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Expression profile and estrogenic regulation of Amh during gonadal sex differentiation in northern snakehead (Channa argus)

Genes & Genomics 2020년 42권 7호 p.827 ~ 835
 ( Luo Qing ) - Chinese Academy of Fishery Sciences Pearl River Fisheries Research Institute

 ( Ou Mi ) - Chinese Academy of Fishery Sciences Pearl River Fisheries Research Institute
 ( Zhao Jian ) - Chinese Academy of Fishery Sciences Pearl River Fisheries Research Institute
 ( Liu Haiyang ) - Chinese Academy of Fishery Sciences Pearl River Fisheries Research Institute
 ( Gao Dandan ) - Chinese Academy of Fishery Sciences Pearl River Fisheries Research Institute
 ( Wu Yanduo ) - Chinese Academy of Fishery Sciences Pearl River Fisheries Research Institute
 ( Zhang Lanlan ) - Sun Yat-Sen University Zhongshan School of Medicine
 ( Chen Kunci ) - Chinese Academy of Fishery Sciences Pearl River Fisheries Research Institute

Abstract


Background: Anti-Mullerian hormone (Amh) plays a critical role in both early sex determination and later gonad development in vertebrate species. However, it remains unknown in northern snakehead (Channa argus), which is economically important freshwater fish with sexual dimorphism.

Objective: This study aimed to identify the expression profiles and estrogenic regulation of CaAmh during gonadal sex differentiation in C. argus.

Methods: The cDNA and genomic DNA sequences of CaAmh were identified by PCR and RACE techniques. The expression patterns of CaAmh were detected by qRT-PCR during the gonadal sex differentiation and after 17α-ethinyloestradiol (EE2) treatments.

Results: CaAmh is composed of seven exons and six introns, and its full-length cDNA is 2413 bp in length, with 1635 bp open reading frame (ORF) that encodes a 544 amino acid protein. Tissues expression patterns revealed that CaAmh display the highest expression in testis of XY males (40.36 folds, p?
Conclusions: These results show that Amh plays a critical role in testicular differentiation of C. argus and it is apparently modulated by estrogens in this species.

키워드

Channa argus; Amh; Estrogen; Sex differentiation
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