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Identifying genes for resistant starch, slowly digestible starch, and rapidly digestible starch in rice using genome-wide association studies

Genes & Genomics 2020년 42권 11호 p.1227 ~ 1238
Zhang Ning, Wang Maike, Fu Ji, Shen Yi, Ding Yi, Wu Dianxing, Shu Xiaoli, Song Wenjian,
소속 상세정보
 ( Zhang Ning ) - Zhejiang University Institute of Nuclear Agricultural Sciences
 ( Wang Maike ) - Zhejiang University Institute of Nuclear Agricultural Sciences
 ( Fu Ji ) - Zhejiang University Institute of Nuclear Agricultural Sciences
 ( Shen Yi ) - Zhejiang University Institute of Nuclear Agricultural Sciences
 ( Ding Yi ) - Zhejiang University Institute of Nuclear Agricultural Sciences
 ( Wu Dianxing ) - Zhejiang University Institute of Nuclear Agricultural Sciences
 ( Shu Xiaoli ) - Zhejiang University Institute of Nuclear Agricultural Sciences
 ( Song Wenjian ) - Zhejiang University Institute of Nuclear Agricultural Sciences

Abstract


Background: The digestibility of starch is important for the nutritive value of staple food. Although several genes are responsible for resistant starch (RS) and slowly digestible starch (SDS), gaps persist concerning the molecular basis of RS and SDS formation due to the complex genetic mechanisms of starch digestibility.

Objectives: The objective of this study was to identify new genes for starch digestibility in rice and interprete the genetic mechanisms of RS and SDS by GWAS.

Methods: Genome-wide association studies were conducted by associating the RS and SDS phenotypes of 104 re-sequenced rice lines to an SNP dataset of 2,288,867 sites using a compressed mixed linear model. Candidate genes were identified according to the position of the SNPs based on data from the MSU Rice Genome Annotation Project.

Results: Seven quantitative trait loci (QTLs) were detected to be associated with the RS content, among which the SNP 6 m1765761 was located on Waxy. Starch branching enzymes IIa (BEIIa) close to QTL qRS-I4 was detected and further identified as a specific candidate gene for RS in INDICA. Two QTLs were associated with SDS, and the LOC_Os09g09360 encoding lipase was identified as a causal gene for SDS.

Conclusions: GWAS is a valid strategy to genetically dissect the formation of starch digestion properties in rice. RS formation in grains is dependent on the rice type; lipid might also contribute to starch digestibility and should be an alternative factor to improve rice starch digestibility.

키워드

Rice; Resistant starch; Slowly digestible starch; GWAS

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