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Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus)

Genes & Genomics 2020년 42권 11호 p.1281 ~ 1290
Liang Shuang, Bao Zhiyuan, Zhao Bohao, Zhou Tong, Li Jiali, Liu Ming, Hu Shuaishuai, Yang Naisu, Chen Yang, Wu Xinsheng,
소속 상세정보
 ( Liang Shuang ) - Yangzhou University College of Animal Science and Technology
 ( Bao Zhiyuan ) - Yangzhou University College of Animal Science and Technology
 ( Zhao Bohao ) - Yangzhou University College of Animal Science and Technology
 ( Zhou Tong ) - Yangzhou University College of Animal Science and Technology
 ( Li Jiali ) - Yangzhou University College of Animal Science and Technology
 ( Liu Ming ) - Yangzhou University College of Animal Science and Technology
 ( Hu Shuaishuai ) - Yangzhou University College of Animal Science and Technology
 ( Yang Naisu ) - Yangzhou University College of Animal Science and Technology
 ( Chen Yang ) - Yangzhou University College of Animal Science and Technology
 ( Wu Xinsheng ) - Yangzhou University College of Animal Science and Technology

Abstract


Background: Keratin-associated protein (KAP), the structural protein molecule of hair fibers, plays a key role in determining the physical properties of hair. Studies of Krtap11-1 have focused only on its localization. Functional studies of Krtap11-1 in hair follicle development have so far not been reported.

Objective: This study aimed to provide evidence for the role of Krtap11-1 in skin and hair development.

Methods: Full-length cloning and analysis of Krtap11-1 were conducted to ascertain its function. Overexpression vectors and interference sequences were constructed and transfected into RAB-9 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate the hair follicle developmental stage of Krtap11-1, the expression of different tissues, and the effects on other hair follicle development-related genes.

Results: The full length of cloned Krtap11-1 was 947 bp. Krtap11-1 was confirmed to be a hydrophilic protein localized mostly in mitochondria. The greatest mRNA expression was observed in skin. Using a follicle synchronization model, it was found that Krtap11-1 mRNA expression levels first increased then decreased over the passage of time, principally during hair follicle catagen and telogen. Following the overexpression of Krtap11-1, mRNA expression levels of the WNT-2, KRT17, BMP-2, and TGF-β-1 genes increased, and LEF-1 decreased (P?
Conclusions: Krtap11-1 exerts a promoting effect. The results provide novel insight into the relationship between hair follicle development and Krtap11-1 gene expression.

키워드

Krtap11-1; Angora rabbit; Hair follicle development; Expression analysis

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