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Differential expression profile of microRNA in yak skeletal muscle and adipose tissue during development

Genes & Genomics 2020년 42권 11호 p.1347 ~ 1359
Ji Hui, Wang Hui, Ji Qiumei, Ji Winbo, Luo Xiaolin, Wang Jiabo, Chai Zhixin, Xin Jinwei, Cai Xin, Wu Zhijuan, Wang Jikun, Zhong Jincheng,
소속 상세정보
 ( Ji Hui ) - Southwest Minzu University
 ( Wang Hui ) - Southwest Minzu University
 ( Ji Qiumei ) - Tibet Academy of Agriculture and Animal Husbandry
 ( Ji Winbo ) - Southwest Minzu University
 ( Luo Xiaolin ) - Sichuan Grassland Science Academy
 ( Wang Jiabo ) - Southwest Minzu University
 ( Chai Zhixin ) - Southwest Minzu University
 ( Xin Jinwei ) - Tibet Academy of Agriculture and Animal Husbandry
 ( Cai Xin ) - Southwest Minzu University
 ( Wu Zhijuan ) - Southwest Minzu University
 ( Wang Jikun ) - Southwest Minzu University
 ( Zhong Jincheng ) - Southwest Minzu University

Abstract


Background: miRNAs play an important role in regulating normal animal development. Muscle tissue and fat metabolism are important for maintaining energy balance in animals. Yak has important agricultural and economic importance as it provides milk, meat, and hair. It is used for transportation as well. However, the miRNA expression profiles of their muscle and adipose tissue are currently unknown.

Objective: To explore the regulatory roles of miRNAs in the skeletal muscle and adipose tissues of yak.

Methods: A total of 12 small RNA libraries were constructed from the skeletal muscle and adipose samples from yak aged 0.5, 2.5, 4.5, and 7.5 years. High-throughput sequencing and bioinformatics analysis were used to determine the dynamic expression profile of miRNA, and a miRNA regulatory network related to muscle and adipose tissue development was established.

Results: miR-1-3p and miR-143-3p showed the highest expression during yak skeletal muscle and fat development, respectively. The MAPK and Ras signaling pathways were the pivotal pathways. miR-181-5p, miR-542-3p, and miR-424-5p may have key roles in skeletal muscle development, and CREBRF, GRB10, CDK1, RFX3, and EPC2 were the core target genes. While miR-127-5p, miR-379-3p, and miR-494-3p may play important regulatory roles in adipose deposition, and ETV1, XPO7, and C5AR2 were the core target genes.

Conclusion: This study provides valuable resources for further study of the molecular mechanisms underlying yak skeletal muscle and adipose tissue development, and also a basis for studying the interactions between genes and miRNAs.

키워드

Yak; Skeletal muscle; Adipose; miRNA

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