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SsPsaH, a H subunit of the photosystem I reaction center of Suaeda salsa, confers the capacity of osmotic adjustment in tobacco

Genes & Genomics 2020년 42권 12호 p.1455 ~ 1465
Hao Xiaoyan, Li Jianping, Gao Shengqi, Tuerxun Zumuremu, Chang Xiaochun, Hu Wenran, Chen Guo, Huang Quansheng,
소속 상세정보
 ( Hao Xiaoyan ) - Xinjiang Academy of Agricultural Sciences Institute of Nuclear and Biological Technology
 ( Li Jianping ) - Xinjiang Academy of Agricultural Sciences Institute of Nuclear and Biological Technology
 ( Gao Shengqi ) - Xinjiang Academy of Agricultural Sciences Institute of Nuclear and Biological Technology
 ( Tuerxun Zumuremu ) - Xinjiang Academy of Agricultural Sciences Institute of Nuclear and Biological Technology
 ( Chang Xiaochun ) - Xinjiang Academy of Agricultural Sciences Institute of Nuclear and Biological Technology
 ( Hu Wenran ) - Xinjiang Academy of Agricultural Sciences Institute of Nuclear and Biological Technology
 ( Chen Guo ) - Xinjiang Academy of Agricultural Sciences Institute of Nuclear and Biological Technology
 ( Huang Quansheng ) - Xinjiang Academy of Agricultural Sciences Institute of Nuclear and Biological Technology

Abstract


Background: Abiotic stress effects agricultural production, so research on improving stress tolerance of crop is important. Suaeda salsa is a halophyte with high salt and drought tolerance and ability to desalinate saline soil and improve soil quality.

Objective: To discover and utilize of salt and drought tolerance-related genes, we further investigated the mechanisms of salt and drought tolerance.

Methods: Through screening a salt treated Suaeda salsa cDNA library and further cloning a H subunit of the photosystem I reaction center SsPsaH cDNA, and then the protein domain and phylogenetic analyses of PSI genes was conducted with the NCBI Blast, DNAMAN, and MotifScan programs. The S. salsa seedlings were subjected to various stress treatments and analyze expression of SsPsaH under these treatments by real-time RT-PCR. SsPsaH expression construct was introduced into S. pombe cells by electroporation and transformed into N. tabacum plants by the leaf disc transformation method.

Results: A member of the H subunit of the Photosystem I reaction center (defined as SsPsaH) was obtained. The expression of SsPsaH was up-regulated by abscisic acid (ABA), salt, and drought stress treatments. Over-expressing SsPsaH in recombinant yeasts enhanced high salinity tolerance and increased tolerance to sorbitol during seed germination and seedling root development in tobacco, respectively. Some stress-related mark genes such as a LEA family gene of NtLEA, a binding protein of a drought response element of NtDREB, the ascorbate peroxidase gene (NtAPX) were also up-regulated in SsPsaH overexpressing transgenic tobacco lines.

Conclusions: These results show that SsPsaH may contribute to the salt and osmotic stress response of plants.

키워드

Photosystem I; The H subunit of the Photosystem I reaction center(PsaH); Suaeda salsa; Stress tolerance

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