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The impact of freeze-drying on the glycoproteomic profiles of human milk

분석과학 2020년 33권 4호 p.177 ~ 185
한원호, 배성필, 이후근, 박종문, 박수연, 이주현, 강남미,
소속 상세정보
한원호 ( Hahn Won-Ho ) - Soonchunhyang University School of Medicine Department of Pediatrics
배성필 ( Bae Seong-Phil ) - Soonchunhyang University Seoul Hospital Department of Pediatrics
이후근 ( Lee Hoo-Keun ) - Gachon University College of Pharmacy
박종문 ( Park Jong-Moon ) - Gachon University College of Pharmacy
박수연 ( Park Su-Yeon ) - Soonchunhyang Medical Center Department of Biostatistics
이주현 ( Lee Joo-Hyun ) - Konkuk University Department of Crop Science
강남미 ( Kang Nam-Mi ) - Konkuk University Department of Nursing

Abstract


Human milk (HM) glycoproteins play important roles protecting infants against various pathogens.
Recently, freezing HM is reported to affect some glycoproteins and freeze-drying is suggested as an alternative method. However, the effects of freeze-drying on HM glycoproteins were not evaluated yet. Six fresh HM samples were collected from three healthy mothers at 15 and 60th days of lactation from each mother. Each sample was divided into frozen and freeze-dried subgroups yielding totally 12 samples, and the glycoproteomic analysis was performed by liquid chromatography mass spectrometry. The results were compared between samples of 15 and 60th days of lactation, and before and after the freeze-drying. Totally, 203 glycoproteins were detected. The glycoprotein levels were not different between two groups of 15/60th day of lactation and before/after freeze-drying groups (P > 0.050). In addition, significant correlation of glycoprotein levels was found between the different lactation stages (r = 0.897, P < 0.001) and the status of freeze-drying (r = 0.887, P < 0.001) in a partial correlation analysis. As no significant change of HM glycoproteins was not found after the freezedrying, we hope that introducing freeze-drying to HM banks is supported by the present study. This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Korea government (MSIP) (No.2017R1D1A1B03034270; No.2020R1A2C1005082).

키워드

freeze-drying; human milk; lyophilization; mass spectrometry; glycoprotein; glycoproteomics

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