잠시만 기다려 주세요. 로딩중입니다.

A study on the analysis of artificial sweeteners in processed foods including emulsified foods by HPLC-DAD

분석과학 2020년 33권 5호 p.209 ~ 214
김민희, 김도연, 안장혁,
소속 상세정보
김민희 ( Kim Min-Hee ) - Fore Front TEST Department of Research & Development
김도연 ( Lee Do-Yeon ) - Fore Front TEST Department of Research & Development
안장혁 ( Ahn Jang-Hyuk ) - Fore Front TEST Department of Research & Development

Abstract


This study has been carried out to develop a standard method for quantifying of 3 permitted artificial sweeteners (including sodium saccharine, aspartame, acesulfame potassium) contained in foods by HPLC-DAD.
A simple and rapid sample pretreatment method was used to remove fat and protein from the test solution with Carrez clearing regent precipitant know to be effective for protein and fat removal. The artificial sweeteners in the test solution purified through sample pretreatment were detected by high performance liquid chromatograph using a Reverse phase C18 column (5 μm, 4.6 × 250 mm). The simultaneous quantitative test of 3 kinds of artificial sweeteners can be effectively performed on the high fat emulsified foods containing a large amount of fat. Using the established simultaneous quantitative test method, artificial sweeteners were tested in foods such as dairy products, snacks and chocolate. The results calibration curve showed good linearity with high regression coefficients and the result of recovery test showed satisfactory recoveries within 80~110 %.

키워드

artificial sweeteners; simultaneous analysis; fat and emulsified food; high performance liquid chromatograph; food additive

원문 및 링크아웃 정보

등재저널 정보

KCI