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Simultaneous qualitative and quantitative analysis of morroniside and hederacoside D in extract mixture of Cornus officinalis and Stauntonia hexaphylla leaves to improve benign prostatic hyperplasia by HPLC-UV

분석과학 2020년 33권 5호 p.224 ~ 231
Dan Gao, 조종운, Vinh Le Ba, 김진혁, 조경원, 김영호, 강종성,
소속 상세정보
 ( Dan Gao ) - Chungnam National University College of Pharmacy
조종운 ( Cho Chong-Woon ) - Chungnam National University College of Pharmacy
 ( Vinh Le Ba ) - Chungnam National University College of Pharmacy
김진혁 ( Kim Jin-Hyeok ) - Chungnam National University College of Pharmacy
조경원 ( Cho Kyoung-Won ) - Chong Kun Dang Healthcare
김영호 ( Kim Young-Ho ) - Chungnam National University College of Pharmacy
강종성 ( Kang Jong-Seong ) - Chungnam National University College of Pharmacy

Abstract


With the improvement in the standard of living and extension of life expectancy, the incidence of prostate diseases has increased yearly, thus becoming a serious disease affecting the health of men. The extract mixture of Cornus officinalis and Stauntonia hexaphylla leaves is a developed functional food formula to improve prostate health. This study developed a simultaneous analytical method of bioactive compounds for quantifying the mixture of Cornus officinalis and S. hexaphylla leaves using high-pressure liquid chromatography-ultraviolet (HPLC?UV). HPLC analytical condition was performed on a Hector C18 column with a mobile phase of 0.1 % formic acid in water (A) and 0.1 % formic acid in acetonitrile (B) under the following gradient conditions: 0-50 min, 12 %-40 % (B) at a flow rate of 1.0 mL/min. Meanwhile, this method was validated properly and successfully used to quantify the bioactive components of morroniside and hederacoside D in 20 sample batches and assess the quality of different ages and seasons of S. hexaphylla leaves. The result showed that the content of morroniside in the extract mixture of Cornus officinalis and S. hexaphylla leaves ranged from 1.38-1.62 mg/g, and the hederacoside D ranged from 28.42-32.02 mg/g, suggesting that this novel analytical method will be suitable for the quality control of the extract mixture to improve benign prostatic hyperplasia.

키워드

benign prostatic hyperplasia; HPLC-UV; cornus officinalis; stauntonia hexaphylla; LC-ESI-MS/MS

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