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산수유 추출조건에 따른 Loganin 함량 분석 및 활성 평가

Loganin Content and Physiological Activities of Cornus officinalis Extracts Prepared with Different Methods

한국키틴키토산학회지 2020년 25권 3호 p.109 ~ 116
임소연, 정경옥, 이정호,
소속 상세정보
임소연 ( Im So-Yeon ) - Sunchang Research Institute of Health and Longevity
정경옥 ( Jeong Kyoung-Ok ) - Sunchang Research Institute of Health and Longevity
이정호 ( Lee Jeong-Ho ) - Sunchang Research Institute of Health and Longevity

Abstract

수유는 인체 건강에 유익하여 전북지역에서 재배가 증가하고 있어 기능성식품을 개발하고자 loganin의 함량과 항산화, 항당뇨 및 항염 활성, 일반성분을 분석하였다. 산수유에 함유된 loganin 함량을 분석한 결과 3시간, 100℃ 추출물에서 7.68 ± 0.06 mg/g, 9시간 100℃ 추출물에서 7.88 ± 0.04 mg/g 분석되었으며, 추출시간 및 추출온도가 증가함에 따라 loganin 함량도 증가하였다. 산수유 100oC 3시간 추출물 1,000 μg/mL에서 DPPH 라디칼 소거활성은 53.58 ± 3.35%, ABTS 라디칼 소거활성은 18.22 ± 0.62%, SOD 효소 활성은 69.6 ± 7.42%으로 측정되었으며, 추출물의 처리농도가 증가함에 따라 항산화 활성도 증가하였다. 세포 독성은 나타나지 않았으며, NO 생성 저해 활성은 1 μg/mL에서 12.88 ± 0.24, 10 μg/mL 12.89 ± 0.96으로 저해되었다. α-Glucosidase 저해 활성은 1,000 μg/mL에서 98.9 ± 5.95%, 500 μg/mL에서 91.9 ± 5.28%으로 분석되었다. 일반 성분은 열량 344.94 kcal/100 g, 탄수화물 80.83% 조단백질 3.29%, 조지방 0.94%, 총당 347.61 mg/g으로 분석되었다. 산수유에는 loganin이 함유되어 있고 항산화, 항암, 항당뇨 활성이 있어 기능성 소재로 개발이 가능할 것으로 판단된다.

The aim of this study was to investigate the potential of Cornus officinals as a content of protocatechuic acid and physiological activities. The analyzed the content of protocatechuic acid of Cornus officinals water extracts according to different extract temperature and time for extraction. It was analyzed as 7.68 ± 0.06 mg/g in the extract at 100℃ for 3 h. Protocatechuic acid content increased as extraction time and temperature increased. The activity of the Cornus officinals water extract at 100℃ for 3 h was measured. Antioxidant activity against the concentration of Cornus officinals water extract 1,000 μg/mL was measured. DPPH radical scavenging activity was measured at 53.58 ± 3.35%, ABTS radical scavenging activity at 18.22 ± 0.62%, and SOD activity at 69.6 ± 7.42%, antioxidant activity also increased as the processing concentration of extracts increased. Cell viability was shown for RAW 264.7 cells, and nitric oxide(NO) production was also inhibited. The α-Glucosidase inhibitory activity was determined to be 98.9 ± 4.45% at concentration of 1000 μg/mL. The nutritional components were analyzed to be 344.94 kcal/100g of calories, 80.83% of carbohydrates, 3.29% of crude protein, 0.94% of crude fat, and 347.61 mg/g of total sugars. Cornus officinals is thought to be developed as a functional food material because it contains protocatechuic acid and has antioxidant activity, anti-inflammatory and antidiabetic activity.

키워드

Cornus officinalis; Loganin; Antioxidant; α-Glucosidase; Nitric oxide

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