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Transcription Analysis of Recombinant Trichoderma reesei HJ-48 to Compare the Molecular Basis for Fermentation of Glucose and Xylose

Journal of Microbiology and Biotechnology 2020년 30권 10호 p.1467 ~ 1479
Huang Jun, Lin Mei, Liang Shijie, Qin Qiurong, Liao Siming, Lu Bo, Wang Qingyan,
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 ( Huang Jun ) - Guangxi Academy of Sciences Guangxi Biomass Engineering Technology Research Center
 ( Lin Mei ) - Guangxi Academy of Sciences Guangxi Biomass Engineering Technology Research Center
 ( Liang Shijie ) - Guangxi Academy of Sciences Guangxi Biomass Engineering Technology Research Center
 ( Qin Qiurong ) - Guangxi Academy of Sciences Guangxi Biomass Engineering Technology Research Center
 ( Liao Siming ) - Guangxi Academy of Sciences Guangxi Biomass Engineering Technology Research Center
 ( Lu Bo ) - Guangxi Academy of Sciences Guangxi Biomass Engineering Technology Research Center
 ( Wang Qingyan ) - Guangxi Academy of Sciences Guangxi Biomass Engineering Technology Research Center

Abstract


Profiling the transcriptome changes involved in xylose metabolism by the fungus Trichoderma reesei allows for the identification of potential targets for ethanol production processing. In the present study, the transcriptome of T. reesei HJ-48 grown on xylose versus glucose was analyzed using nextgeneration sequencing technology. During xylose fermentation, numerous genes related to central metabolic pathways, including xylose reductase (XR) and xylitol dehydrogenase (XDH), were expressed at higher levels in T. reesei HJ-48. Notably, growth on xylose did not fully repress the genes encoding enzymes of the tricarboxylic acid and respiratory pathways. In addition, increased expression of several sugar transporters was observed during xylose fermentation. This study provides a valuable dataset for further investigation of xylose fermentation and provides a deeper insight into the various genes involved in this process.

키워드

Transcription; Trichoderma reesei; fermentation

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