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Lactobacillus casei LC01 Regulates Intestinal Epithelial Permeability through miR-144 Targeting of OCLN and ZO1

Journal of Microbiology and Biotechnology 2020년 30권 10호 p.1480 ~ 1487
Hou Qiuke, Huang Yongquan, Wang Yan, Liao Liu, Zhu Zhaoyang, Zhang Wenjie, Liu Yongshang, Li Peiwu, Chen Xinlin, Liu Fengbin,
소속 상세정보
 ( Hou Qiuke ) - Guangzhou University of Chinese Medicine First Affiliated Hospital Department of Gastroenterology
 ( Huang Yongquan ) - Guangzhou University of Chinese Medicine Second Affiliated Hospital Department of Orthopedics
 ( Wang Yan ) - Guangzhou University of Chinese Medicine First Affiliated Hospital Department of Gastroenterology
 ( Liao Liu ) - Guangzhou University of Chinese Medicine First Affiliated Hospital Department of Gastroenterology
 ( Zhu Zhaoyang ) - Guangzhou University of Chinese Medicine First Affiliated Hospital Department of Gastroenterology
 ( Zhang Wenjie ) - Guangzhou University of Chinese Medicine First Affiliated Hospital Department of Gastroenterology
 ( Liu Yongshang ) - Guangzhou University of Chinese Medicine First Affiliated Hospital Department of Gastroenterology
 ( Li Peiwu ) - Guangzhou University of Chinese Medicine First Affiliated Hospital Department of Gastroenterology
 ( Chen Xinlin ) - Guangzhou University of Chinese Medicine Department of Preventive Medicine and Health Statistics
 ( Liu Fengbin ) - Guangzhou University of Chinese Medicine First Affiliated Hospital Department of Gastroenterology

Abstract


Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which microRNAs and lactobacilli exert their beneficial effects on intestinal epithelial permeability. Hence, to elucidate whether miRNAs and lactobacilli play roles in intestinal epithelial barrier regulation, we compared miRNA expression levels in intestinal epithelial cells (IECs) under Lactobacillus casei (L. casei LC01) treatment. IECs and L. casei LC01 were co-cultured and then subjected to microRNA microarray assay. qRT-PCR, western blot and ELISA were used to detect the expression of occludin (OCLN) and zonula occludens 1 (ZO1/TJP1). The interaction between miRNAs and L. casei LC01 acting in IECs was investigated through transfection of RNA oligoribonucleotides and pcDNA 3.1 plasmid. The results are as follows: 1) L. casei LC01 decreased the expression of miR-144 and FD4 and promoted OCLN and ZO1 expression in IECs; 2) L. casei LC01 enhanced the barrier function of IECs via downregulation of miR-144 and upregulation of OCLN and ZO1; 3) Under L. casei LC01 treatment, OCLN and ZO1 overexpression could partially eliminate the promoting effect of miR-144 on intestinal permeability in IECs. Our results demonstrate that L. casei LC01 regulates intestinal permeability of IECs through miR-144 targeting of OCLN and ZO1. L. casei LC01 can be a possible therapeutic target for managing dysfunction of the intestinal epithelial barrier.

키워드

miR-144; intestinal epithelial permeability; lactobacilli; occludin (OCLN); zonula occludens 1 (ZO1/TJP1)

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