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Biotransformation of Major Ginsenoside Rb1 to Rd by Dekkera anomala YAE-1 from Mongolian Fermented Milk (Airag)

Journal of Microbiology and Biotechnology 2020년 30권 10호 p.1536 ~ 1542
렌친핸드, 조수현, 박영우, 송규용, 남명수,
소속 상세정보
렌친핸드 ( Renchinkhand Gereltuya ) - Chungnam National University College of Agriculture and Life Sciences Department of Animal Biosystem Sciences
조수현 ( Cho Soo-Hyun ) - Chungnam National University College of Pharmacology Department of Pharmacology Science
박영우 ( Park Young-W. ) - Fort Valley State University Agricultural Research Station
송규용 ( Song Gyu-Yong ) - Chungnam National University College of Pharmacology Department of Pharmacology Science
남명수 ( Nam Myoung-Soo ) - Chungnam National University College of Agriculture and Life Sciences Department of Animal Biosystem Sciences

Abstract


Dekkera anomala YAE-1 strain separated from “airag” (Mongolian fermented mare’s milk) produces β-glucosidase, which can convert ginsenoside Rb1 from Panax ginseng. Ginseng-derived bioactive components such as ginsenoside Rb1 have various immunological and anticancer activities. Airag was collected from five different mare milk farms located near Ulaanbaatar, Mongolia. YAE-1 strains were isolated from airag to examine the hydrolytic activities of β-glucosidase on Korean Panax ginseng using an API ZYM kit. Supernatants of selected cultures having β-glucosidase activity were examined for hydrolysis of the major ginsenoside Rb1 at 40°C, pH 5.0. The YAE-1 strain was found to be nearly identical at 99.9% homology with Dekkera anomala DB-7B, and was thus named Dekkera anomala YAE-1. This strain exerted higher β?glucosidase activity than other enzymes. Reaction mixtures from Dekkera anomala YAE-1 showed great capacity for converting ginsenoside Rb1 to ginsenoside Rd. The β-glucosidase produced by Dekkera anomala YAE-1 was able to hydrolyze ginsenoside Rb1 and convert it to Rd during fermentation of the ginseng. The amount of ginsenoside Rd was highly increased from 0 to 1.404 mg/ml in fermented 20% ginseng root at 7 days.

키워드

Fermented mare’s milk; airag; ginsenoside; β-glucosidase; Dekkera anomala YAE-1

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