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Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract

Journal of Microbiology and Biotechnology 2020년 30권 10호 p.1543 ~ 1551
Li Liang, 박영란, Shrestha Saroj Kumar, 조형권, 서윤조,
소속 상세정보
 ( Li Liang ) - Chonbuk National University School of Dentistry Department of Dental Pharmacology
박영란 ( Park Young-Ran ) - Chonbuk National University School of Dentistry Department of Dental Pharmacology
 ( Shrestha Saroj Kumar ) - Chonbuk National University School of Dentistry Department of Dental Pharmacology
조형권 ( Cho Hyoung-Kwon ) - Hanpoong Pharm & Foods Co. Ltd
서윤조 ( Soh Yun-Jo ) - Chonbuk National University School of Dentistry Department of Dental Pharmacology

Abstract


Panax ginseng has a wide range of activities including a neuroprotective effect, skin protective effects, enhanced DNA repairing, anti-diabetic activity, and protective effects against vascular inflammation. In the present study, we sought to discover the inhibitory effects of a mixture of natural products containing Panax ginseng, Ziziphus jujube, Rubi fructus, Artemisiae asiaticae and Scutellaria baicalensis (PZRAS) on osteoclastogenesis and bone remodeling, as neither the effects of a mixture containing Panax ginseng extract, nor its molecular mechanism on bone inflammation, have been clarified yet. PZRAS upregulated the levels of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GSH-R) and glutathione peroxidase (GSH-Px) and reduced malondialdehyde (MDA) in LPS-treated RAW264.7 cells. Moreover, treatment with PZRAS decreased the production of IL-1β and TNF-α. PZRAS also inhibited osteoclast differentiation through inhibiting osteoclastspecific genes like MMP-2, 9, cathepsin K, and TRAP in RANKL-treated RAW264.7 cells. Additionally, PZRAS has inhibitory functions on the RANKL-stimulated activation of ERK and JNK, which lead to a decrease in the expression of NFATc1 and c-Fos. In an in vivo study, bone resorption induced by LPS was recovered by treatment with PZRAS in bone volume per tissue volume (BV/TV) compared to control. Furthermore, the ratio of eroded bone surface of femurs was significantly increased in LPStreated mice compared to vehicle group, but this ratio was significantly reversed in PZRAS-treated mice. These results suggest that PZRAS could prevent or treat disorders with abnormal bone loss.

키워드

PZRAS; antioxidant; anti-inflammatory; osteoclastogenesis; bone loss

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