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Fermentation and Metabolic Pathway Optimization to De Novo Synthesize (2S)-Naringenin in Escherichia coli

Journal of Microbiology and Biotechnology 2020년 30권 10호 p.1574 ~ 1582
Zhou Shenghu, Hao Tingting, Zhou Jingwen,
소속 상세정보
 ( Zhou Shenghu ) - Jiangnan University National Engineering Laboratory for Cereal Fermentation Technology
 ( Hao Tingting ) - Jiangnan University National Engineering Laboratory for Cereal Fermentation Technology
 ( Zhou Jingwen ) - Jiangnan University National Engineering Laboratory for Cereal Fermentation Technology

Abstract


Flavonoids have diverse biological functions in human health. All flavonoids contain a common 2-phenyl chromone structure (C6-C3-C6) as a scaffold. Hence, in using such a scaffold, plenty of highvalue-added flavonoids can be synthesized by chemical or biological catalyzation approaches. (2S)-Naringenin is one of the most commonly used flavonoid scaffolds. However, biosynthesizing (2S)-naringenin has been restricted not only by low production but also by the expensive precursors and inducers that are used. Herein, we established an induction-free system to de novo biosynthesize (2S)-naringenin in Escherichia coli. The tyrosine synthesis pathway was enhanced by overexpressing feedback inhibition-resistant genes (aroGfbr and tyrAfbr) and knocking out a repressor gene (tyrR). After optimizing the fermentation medium and conditions, we found that glycerol, glucose, fatty acids, potassium acetate, temperature, and initial pH are important for producing (2S)-naringenin. Using the optimum fermentation medium and conditions, our best strain, Nar-17LM1, could produce 588 mg/l (2S)-naringenin from glucose in a 5-L bioreactor, the highest titer reported to date in E. coli.

키워드

L-tyrosine; p-coumaric acid; dynamic regulation; flavonoids; temperature-shift

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