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Physiological Response of Escherichia coli W3110 and BL21 to the Aerobic Expression of Vitreoscilla Hemoglobin

Journal of Microbiology and Biotechnology 2020년 30권 10호 p.1592 ~ 1596
Lara Alvaro R., Galindo Janet, Jaen Karim E., Juarez Mariana, Sigala Juan-Carlos,
소속 상세정보
 ( Lara Alvaro R. ) - Universidad Autonoma Metropolitana-Cuajimalpa Departamento de Procesos y Tecnologia
 ( Galindo Janet ) - Universidad Autonoma Metropolitana-Cuajimalpa Departamento de Procesos y Tecnologia
 ( Jaen Karim E. ) - Universidad Autonoma Metropolitana-Cuajimalpa Departamento de Procesos y Tecnologia
 ( Juarez Mariana ) - Universidad Autonoma Metropolitana-Cuajimalpa Departamento de Procesos y Tecnologia
 ( Sigala Juan-Carlos ) - Universidad Autonoma Metropolitana-Cuajimalpa Departamento de Procesos y Tecnologia

Abstract


The aerobic growth and metabolic performance of Escherichia coli strains BL21 and W3110 were studied when the Vitreoscilla hemoglobin (VHb) was constitutively expressed in the chromosome. When VHb was expressed, acetate production decreased in both strains and was nearly eliminated in BL21. Transcriptional levels of the glyoxylate shunt genes decreased in both strains when VHb was expressed. However, higher transcription of the α-ketoglutarate dehydrogenase genes were observed for W3110, while for BL21 transcription levels decreased. VHb expression reduced the transcription of the cytochrome bo3 genes only in BL21. These results are useful for better selecting a production host.

키워드

Vitreoscilla hemoglobin; aerobic cultures; E. coli W3110; BL21; transcriptional analysis

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