High-Throughput In Vitro Screening of Changed Algal Community Structure Using the PhotoBiobox
조대현, 조기철, 허진아, 김우림, 이용재, 최동윤, 유찬, 김희식, 배승희,
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조대현 ( Cho Dae-Hyun ) - Korea Research Institute of Bioscience and Biotechnology Cell Factory Research Center
조기철 ( Cho Ki-Chul ) - National Marine Biodiversity Institute of Korea Department of Genetic Resources Research
허진아 ( Heo Jin-A ) - Korea Research Institute of Bioscience and Biotechnology Cell Factory Research Center
김우림 ( Kim U-Rim ) - Korea Research Institute of Bioscience and Biotechnology Cell Factory Research Center
이용재 ( Lee Yong-Jae ) - Korea Research Institute of Bioscience and Biotechnology Cell Factory Research Center
최동윤 ( Choi Dong-Yun ) - Korea Research Institute of Bioscience and Biotechnology Cell Factory Research Center
유찬 ( Yoo Chan ) - KAIST Department of Chemical and Biomolecular Engineering
김희식 ( Kim Hee-Sik ) - Korea Research Institute of Bioscience and Biotechnology Cell Factory Research Center
배승희 ( Bae Seung-Hee ) - Konkuk University Department of Cosmetics Engineering
Abstract
In a previous study, the sequential optimization and regulation of environmental parameters using the PhotoBiobox were demonstrated with high-throughput screening tests. In this study, we estimated changes in the biovolume-based composition of a polyculture built in vitro and composed of three algal strains: Chlorella sp., Scenedesmus sp., and Parachlorella sp. We performed this work using the PhotoBiobox under different temperatures (10-36°C) and light intensities (50-700 μmol/m-2/s-1) in air and in 5% CO2. In 5% CO2, Chlorella sp. exhibited better adaptation to high temperatures than in air conditions. Pearson’s correlation analysis showed that the composition of Parachlorella sp. was highly related to temperature whereas Chlorella sp. and Scenedesmus sp. showed negative correlations in both air and 5% CO2. Furthermore, light intensity slightly affected the composition of Scenedesmus sp., whereas no significant effect was observed in other species. Based on these results, it is speculated that temperature is an important factor in influencing changes in algal polyculture community structure (PCS). These results further confirm that the PhotoBiobox is a convenient and available tool for performance of lab-scale experiments on PCS changes. The application of the PhotoBiobox in PCS studies will provide new insight into polyculture-based ecology.
키워드
PhotoBiobox; temperature; light; microalgae; polyculture; cultivation
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