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Diosmetin and Its Glycoside, Diosmin, Improve Atopic Dermatitis- Like Lesions in 2,4-Dinitrochlorobenzene-Induced Murine Models

Biomolecules & Therapeutics 2020년 28권 6호 p.542 ~ 558
박상아, 봉심규, 이진우, Park No-June, 최용수, 김상무, 양민혜, 김영기, 김수남,
소속 상세정보
박상아 ( Park Sang-A ) - Korea Institute of Science and Technology Natural Products Research Institute
봉심규 ( Bong Sim-Kyu ) - Korea Institute of Science and Technology Natural Products Research Institute
이진우 ( Lee Jin-Woo ) - Korea Institute of Science and Technology Natural Products Research Institute
 ( Park No-June ) - Korea Institute of Science and Technology Natural Products Research Institute
최용수 ( Choi Yong-Soo ) - Korea Institute of Science and Technology Natural Products Research Institute
김상무 ( Kim Sang-Moo ) - Gangneung-Wonju National University Department of Marine Food Science and Technology
양민혜 ( Yang Min-Hye ) - Pusan National University College of Pharmacy
김영기 ( Kim Yong-Kee ) - Sookmyung Women’s University College of Pharmacy
김수남 ( Kim Su-Nam ) - Korea Institute of Science and Technology Natural Products Research Institute

Abstract


Naturally derived diosmetin and its glycoside diosmin are known to be effective in treating inflammatory disease. This study was performed to determine whether diosmin and diosmetin have the effect of improving atopic dermatitis in a 2,4-dinitrochlorobenzen (DNCB)-induced atopic dermatitis (AD) model. DNCB was used to establish AD model in hairless mice. Skin moisture, serum immunoglobulin E (IgE), interleukin 4 (IL-4), and histological analysis were performed to measure the effectiveness of diosmin and diosmetine to improve AD. IL-4 levels were also measured in RBL-2H3 cells. Administration of diosmetin or diosmin orally inhibited the progress of DNCB-induced AD-like lesions in murine models by inhibiting transdermal water loss (TEWL) and increasing skin hydration. Diosmetin or diosmin treatment also reduced IgE and IL-4 levels in AD-induced hairless mouse serum samples. However, in the in vitro assay, only diosmetin, not diosmin, reduced the expression level of IL-4 mRNA in RBL-2H3 cells. Diosmin and diosmetine alleviated the altered epidermal thickness and immune cell infiltration in AD. Diosmin is considered effective in the cure of AD and skin inflammatory diseases by being converted into diosmetin in the body by pharmacokinetic metabolism. Thus, oral administration of diosmetin and diosmin might be a useful agent for the treatment of AD and cutaneous inflammatory diseases.

키워드

Diosmetin; Diosmin; Atopic dermatitis; IL-4; DNCB

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