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엉겅퀴에 함유된 Cirsimaritin 분석을 위한 HPLC 분석법 밸리데이션

Validation of an HPLC/UV Analysis Method for Cirsimaritin in Cirsium japonicum var. maackii

생약학회지 2020년 51권 3호 p.217 ~ 221
이주성, 레오, 조이스, 강기성, 함대현, 심재숙, 최유진, 이상현,
소속 상세정보
이주성 ( Lee Ju-Sung ) - Chung-Ang University Department of Plant Science and Technology
레오 ( Leo Adrianne Paje ) - Chung-Ang University Department of Plant Science and Technology
조이스 ( Joyce P. Rodriguez ) - University of San Carlos Department of Biology
강기성 ( Kang Ki-Sung ) - Gachon University College of Korean Medicine
함대현 ( Hahm Dae-Hyun ) - Kyung Hee University College of Medicine Department of Physiology
심재숙 ( Shim Jae-Suk ) - Imsil Herbal Medicine Association
최유진 ( Choi Yu-Jin ) - Imsil Research Institute of Cheese Science
이상현 ( Lee Sang-Hyun ) - Chung-Ang University Department of Plant Science and Technology

Abstract


Cirsium japonicum var. maackii (CM) has been used to treat certain medical conditions such as hemorrhage, hepatitis, and hypertension. Cirsimaritin was previously found as the major flavonoid in CM and is said to contribute to its pharmacological effects. There are currently no reports detailing the qualitative and quantitative detection of phytochemical indicators in the aerial parts of CM. Therefore, we developed a method to rapidly identify and quantify cirsimaritin in CM using HPLC/UV, and we optimized and validated this analytical method. The results showed good linearity in the concentration range tested (0.25-0.015 mg/mL, r2 ³ 0.9999), accuracy (93.9-111.3%), and precision (RSD £ 0.59%). The developed method can therefore be used for the rapid evaluation of cirsimaritin in CM.

키워드

Cirsium japonicum var; maackii; Cirsimaritin; HPLC/UV; Method validation

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