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Effects of Compounds from Physalis angulata on Fatty Acid Synthesis and Glucose Metabolism in HepG2 Cells via the AMP-activated Protein Kinase Pathway

Natural Product Sciences 2020년 26권 3호 p.200 ~ 206
Hoa Hoang Thai, Thu Nguyen Thi, Dong Nguyen Thuong, Oanh Tran Thi, Hien Tran Thi, Ha Do Thi,
소속 상세정보
 ( Hoa Hoang Thai ) - National Institute of Medical Materials
 ( Thu Nguyen Thi ) - National Institute of Medical Materials
 ( Dong Nguyen Thuong ) - National Institute of Medical Materials
 ( Oanh Tran Thi ) - Institute for Research and Development of Organic Products
 ( Hien Tran Thi ) - Lund University Institute of Laboratory Medicine
 ( Ha Do Thi ) - National Institute of Medical Materials

Abstract


The ability of the total extract from Physalis angulata; three fractions after partitioning with nhexane, ethyl acetate (TBE), and water; and four withanolides (compounds 1 - 4) to phosphorylate 5'-adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in HepG2 cells was evaluated. The TBE fraction (50 μg/mL) activated p-ACC and p-AMPK expression most strongly. Compounds 1 - 4 (10 μM) upregulated p-ACC expression at different levels. Compound 4 induced the most significant changes in p-AMPK expression, followed by 1 and 2. Sterol regulatory element-binding proteins (SREBPs) play a functional role in the transcriptional regulation of the lipogenic pathway, including fatty acid synthase (FAS) and ACC. The effects of compounds 2 and 4 (10 μM) on FAS and SREBP-1c expression under high glucose conditions (30 mM) in HepG2 cells were evaluated further. Both dose-dependently inhibited FAS and SREBP-1c expression as well as lipid accumulation (1 - 10 μM) were compared to high-concentration glucose control, which upregulated FAS and SREBP-1c. These results suggest that compounds 2 and 4 upregulate AMPK, suppress FAS and SREBP-1c, and have potential effects on glucose and lipid metabolism.

키워드

Physalis angulata; Withanolides; AMPK; ACC; FAS; SREBP-1c

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