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Isolation and Identification of Bioactive Compounds from the Tuber of Brassica oleracea var. gongylodes

Natural Product Sciences 2020년 26권 3호 p.214 ~ 220
Prajapati Ritu, 성수희, 김형락, 정현아, 최재수,
소속 상세정보
 ( Prajapati Ritu ) - Pukyong National University Department of Food and Life Science
성수희 ( Seong Su-Hui ) - Pukyong National University Department of Food and Life Science
김형락 ( Kim Hyeung-Rak ) - Pukyong National University Department of Food and Life Science
정현아 ( Jung Hyun-Ah ) - Chonbuk National University Department of Food Science and Human Nutrition
최재수 ( Choi Jae-Sue ) - Pukyong National University Department of Food and Life Science

Abstract


Brassica oleracea var. gongylodes (red kohlrabi) is a biennial herbaceous vegetable whose edible bulbotuber-like stem and leaves are consumed globally. Sliced red kohlrabi tubers were extracted using methanol and the concentrated extract was partitioned successively with dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (n-BuOH) and water (H2O). Repeated column chromatography of EtOAc fraction through silica, sephadex LH-20 and RP-18 gel led to isolation of eleven compounds of which compound 1 was a new glycosylated indole alkaloid derivative, 1-methoxyindole 3-carboxylic acid 6-O-β-D-glucopyranoside. Others were known compounds namely, β-sitosterol glucoside (4), 5-hydroxymethyl-2-furaldehyde (5), methyl-1-thio-β- D-glucopyranosyl disulfide (6), 5-hydroxy-2-pyridinemethanol (7), (3S,4R)-2-deoxyribonolactone (8), n-butyl-β- D-fructopyranoside (9), uridine (10) and three fructose derivatives, D-tagatose (11), β-D-fructofuranose (12) and β-D-fructopyranose (13). Similarly, isolation from CH2Cl2 fraction gave two known indole alkaloids, indole 3- acetonitrile (2) and N-methoxyindole 3-acetonitrile (3). The structure elucidation and identification of these compounds were conducted with the help of 13C and 1H NMR, HMBC, HMQC, EIMS, HR-ESIMS and IR spectroscopic data, and TLC plate spots visualization. Compounds 2, 3, 4, 5, 6, 7, 8 and 9 are noted to occur in kohlrabi for the first time. Different bioactivities of these isolated compounds have been reported in literature.

키워드

Brassica oleracea var; gongylodes; 1-methoxyindole 3-carboxylic acid 6-O-β-D-glucopyranoside; bioactive compounds

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