잠시만 기다려 주세요. 로딩중입니다.

Anti-adipogenic Pregnane Steroid from a Hydractinia-associated Fungus, Cladosporium sphaerospermum SW67

Natural Product Sciences 2020년 26권 3호 p.230 ~ 235
이성락, 강희선, 유민정, 이상아, Beemelmanns Christine, 이재철, 김기현,
소속 상세정보
이성락 ( Lee Seoung-Rak ) - Sungkyunkwan University School of Pharmacy
강희선 ( Kang Hee-Sun ) - Sungkyunkwan University School of Pharmacy
유민정 ( Yoo Min-Jeong ) - Sungkyunkwan University School of Pharmacy
이상아 ( Yi Sang-Ah ) - Sungkyunkwan University School of Pharmacy
 ( Beemelmanns Christine ) - Hans Knoll Institute Leibniz Institute for Natural Product Research and Infection Biology
이재철 ( Lee Jae-Cheol ) - Sungkyunkwan University School of Pharmacy
김기현 ( Kim Ki-Hyun ) - Sungkyunkwan University School of Pharmacy

Abstract


A pregnane steroid, 3α-hydroxy-pregn-7-ene-6,20-dione (1), was isolated from a Hydractiniaassociated Cladosporium sphaerospermum SW67 by repetitive column chromatographic separation and highperformance liquid chromatography (HPLC) purification. The planar structure of 1 was elucidated from the analysis of the spectroscopic data (1D and 2D NMR spectra) and LC-MS data. The absolute configuration of 1 was determined by interpretation of ROESY spectrum of 1, together with the comparison of reported spectroscopic values in previous studies. To the best of our knowledge, this is the first report of the identification of the pregnane scaffold from C. sphaerospermum, a natural source. Compound 1 was evaluated for its effects on lipid metabolism and adipogenesis during adipocyte maturation and showed that compound 1 substantially inhibited lipid accumulation compared to the control. Consistently, the expression of the adipocyte marker gene (Adipsin) was reduced upon incubation with 1. Further, we evaluated the effects of 1 on lipid metabolism by measuring the transcription of lipolytic and lipogenic genes. The expression of the lipolytic gene ATGL was significantly elevated upon exposure to 1 during adipogenesis, whereas the expression of lipogenic genes FASN and SREBP1 was significantly reduced upon treatment with 1. Thus, our findings provide experimental evidence that the steroid derived from Hydractinia-associated C. sphaerospermum SW67 is a potential therapeutic agent for obesity.

키워드

Cladosporium sphaerospermum; Hydractinia-associated fungus; pregnane steroid; 3T3-L1 preadipocytes

원문 및 링크아웃 정보

등재저널 정보