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Isolation of Phytochemicals from Salvia plebeia Using Countercurrent Chromatography Coupled with Reversed-phase HPLC

Natural Product Sciences 2020년 26권 3호 p.236 ~ 243
길현우, 노태웅, 서영주, 유아람, 윤기동,
소속 상세정보
길현우 ( Kil Hyun-Woo ) - Catholic University College of Pharmacy
노태웅 ( Rho Tae-Woong ) - Catholic University College of Pharmacy
서영주 ( Seo Young-Ju ) - Catholic University College of Pharmacy
유아람 ( Yu A-Ram ) - Food Industry Promotional Agency of Korea
윤기동 ( Yoon Kee-Dong ) - Catholic University College of Pharmacy

Abstract


Salvia plebeia R. Br. is a plant which has been used as an edible crop and traditional medicine in Asian countries. In this study, HPLC-PDA analysis and countercurrent chromatography (CCC) coupled with reversed-phase (RP) HPLC method were applied to isolate ten isolates from 3.3 g of n-butanol soluble extract from hot-water extract of S. plebeia. The use of CCC enabled us to efficiently fractionate the starting material with less sample loss and facilitate the isolation of compounds from S. plebeia extract using RP-HPLC. The isolates were determined to be caffeic acid (1), 6-hydroxyluteolin 7-O-β-D-glucoside (2), eudebeiolide B (3), (R)- rosmarinic acid (4), homoplantaginin (5), eudebeiolide D (6), plebeiolide C (7), salpleflavone (8), eupafolin (9) and hispidulin (10) based on the spectroscopic evidence.

키워드

Salvia plebeia; Lamiaceae; Countercurrent chromatography; Reversed-phase HPLC

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