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Induction of apopotosis by methanol extracts of Ficus carica L. in FaDu human hypopharynx squamous carcinoma cells

International Journal of Oral Biology 2020년 45권 3호 p.99 ~ 106
이슬아, 박보람, 김춘성,
소속 상세정보
이슬아 ( Lee Seul-Ah ) - Chosun University College of Dentistry Department of Oral Biochemistry
박보람 ( Park Bo-Ram ) - Kyungwoon University College of Health and Welfare Department of Dental Hygiene
김춘성 ( Kim Chun-Sung ) - Chosun University College of Dentistry Department of Oral Biochemistry

Abstract


Ficus carica L. (fig ) is one of the first cultivated crops and is as old as humans. This plant has been extensively usedas a traditional medicine for treating diseases, such as cough, indigestion, nutritional anemia, and tuberculosis. However, the physiological activity of fig leaves on oral cancer is as yet unknown. In this study, we investigatedthe anticancer effect of methanol extracts of Ficus carica (MeFC) and the mechanism of cell death in human FaDuhypopharyngeal squamous carcinoma cells. MeFC decreased the viability of oral cancer (FaDu) cells but did notaffect the viability of normal (L929) cells, as determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide assay and Live and Dead assay. In addition, MeFC induced apoptosis through the proteolytic cleavageof procaspase-3, -9, poly (ADP-ribose) polymerase (PARP), downregulation of Bcl-2, and upregulation of Bax,as determined by 4′,6-diamidino-2-phenylindole dihydrochloride staining and western blot analysis. Moreover, aconcentration of MeFC without cytotoxicity (0.25 mg/mL) significantly suppressed colony formation, a hallmark ofcancer development, and completely inhibited the colony formation at 1 mg/mL. Collectively, these results suggestthat MeFC exhibits a potent anticancer effect by suppressing the growth of oral cancer cells and colony formation viacaspase- and mitochondrial-dependent apoptotic pathways in FaDu human hypopharyngeal squamous carcinomacells. Therefore, the methanol extract of Ficus carcica leaves provide a natural chemotherapeutic drug for human oralcancer.

키워드

Oral cancer; Ficus carica; Apoptosis; Cell growth inhibition

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