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대황(大黃)과 황금(黃芩) 추출물 혼합물이 급성 역류성 식도염 흰쥐에 미치는 효과

Effect of a Mixture of Rhei Rhizoma and Scutellariae Radix Extract on Acute Reflux Esophagitis Rats

대한본초학회지 2020년 35권 6호 p.43 ~ 53
이진아, 신미래, 이상남, 박순애, 박해진,
소속 상세정보
이진아 ( Lee Jin-A ) - Daegu Haany University College of Korean Medicine Department of Herbology
신미래 ( Shin Mi-Rae ) - Daegu Haany University College of Korean Medicine Department of Herbology
이상남 ( Lee Sang-Nam ) - Daegu Haany University College of Korean Medicine Department of Qigong
박순애 ( Park Soon-Ae ) - Daegu Haany University Department of Herbal Food Cuisine & Nutrition
박해진 ( Park Hae-Jin ) - DHU Bio Convergence Testing Center

Abstract


Objective : Reflux esophagitis is a disease caused by reflux of stomach contents, stomach acid, and pepsin into the esophagus, and is currently increasing worldwide. This study was conducted to evaluate the effect of a mixture of Rhei Rhizoma and Scutellariae Radix (RS) extract on acute reflux esophagitis in rats.

Methods : Rats were divided into five groups for examination: Normal group (Nor, n=8), water-treated acute reflux esophagitis rats (Con, n=8), tocopherol 30 ㎎/㎏ body weight/day-treated acute reflux esophagitis rats (Toco, n=8), RS 100 ㎎/㎏ body weight/day-treated acute reflux esophagitis rats (RS100, n=8), RS 200 ㎎/㎏ body weight/day-treated acute reflux esophagitis rats (RS200, n=8). All rats fasted for 18 h and then were derived by linking the metastatic junction between pylorus and forestomach and corpus. And rats were sacrificed 5 h after surgery. We analyzed the expression of NADPH, MAPK, inflammatory, anti-inflammatory, and tight junction related proteins by western blot in esophageal tissue and observed the level of reactive oxygen species (ROS), alanine aminotransferanse (ALT), and aspartate aminotransferase (AST) in serum.

Results : RS administration significantly protected the esophageal mucosal damage of reflux esophagitis, and ROS, AST, and ALT levels were significantly reduced in RS administration compared to Con group. In addition, RS administration effectively suppressed MAPK and NF-κB pathways and upregulated protein expressions of tight junction protein.
Conclusions : These results suggest that RS protected the esophageal mucosa by inhibiting the MAPK and NF-κB pathways and upregulating tight junctions.

키워드

Rhei Rhizoma; Scutellariae Radix; acute reflux esophagitis; inflammation; tight junction

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