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Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina

Journal of Microbiology 2020년 58권 12호 p.988 ~ 997
Chua Kah-Ooi, See-Too Wah-Seng, Tan Jia-Yi, Song Sze-Looi, Yong Hoi-Sen, Yin Wai-Fong, Chan Kok-Gan,
소속 상세정보
 ( Chua Kah-Ooi ) - University of Malaya Faculty of Science Institute of Biological Sciences
 ( See-Too Wah-Seng ) - University of Malaya Faculty of Science Institute of Biological Sciences
 ( Tan Jia-Yi ) - University of Malaya Faculty of Science Institute of Biological Sciences
 ( Song Sze-Looi ) - University of Malaya Faculty of Science Institute of Biological Sciences
 ( Yong Hoi-Sen ) - University of Malaya Faculty of Science Institute of Biological Sciences
 ( Yin Wai-Fong ) - University of Malaya Faculty of Science Institute of Biological Sciences
 ( Chan Kok-Gan ) - University of Malaya Faculty of Science Institute of Biological Sciences

Abstract


In this study, bacterial strains Ha5T, Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15?37°C (optimum, 28?30°C) and in the presence of 0?1.5% (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were C18:1ω7c, C16:0, C19:0ω8c cyclo, C14:0, and C16:0 2-OH. Strains Ha5T, Ta1, and Jb2 shared highest 16S rRNA gene sequence similarity (94.56?94.63%) with Neokomagataea tanensis NBRC106556T of family Acetobacteraceae. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (< 71%), in silico DNA-DNA hybridization (< 38%) and average amino acid identity (< 67%) values with all the type members of the family. Based on these results, bacterial strains Ha5T, Ta1, and Jb2 represent a novel species of a novel genus in family Acetobacteaceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5T as the type strain.

키워드

Oecophyllibacter saccharovorans; Acetobacteraceae; insect; phylogenomics; percentage of conserved protein

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