잠시만 기다려 주세요. 로딩중입니다.

Isolation and molecular characterizations of canine distemper virus from a naturally infected Korean dog using Vero cells expressing dog signaling lymphocyte activation molecule

Journal of Veterinary Science 2020년 21권 5호 p.64 ~ 64
양동군, 김하현, 이시우, 윤윤석, 박중원, Oh Dong-Ryul, 유재영, 지미련, Han Bok-Hee, 오수빈, 현방훈,
소속 상세정보
양동군 ( Yang Dong-Kun ) - Animal and Plant Quarantine Agency
김하현 ( Kim Ha-Hyun ) - Animal and Plant Quarantine Agency
이시우 ( Lee Si-U ) - Animal and Plant Quarantine Agency
윤윤석 ( Yoon Yoon-Seek ) - Animal and Plant Quarantine Agency
박중원 ( Park Jung-Won ) - Animal and Plant Quarantine Agency
 ( Oh Dong-Ryul ) - Animal and Plant Quarantine Agency
유재영 ( Yoo Jae-Young ) - Animal and Plant Quarantine Agency
지미련 ( Ji Mi-Ryeon ) - Animal and Plant Quarantine Agency
 ( Han Bok-Hee ) - Animal and Plant Quarantine Agency
오수빈 ( Oh Su-Bin ) - Animal and Plant Quarantine Agency
현방훈 ( Hyun Bang-Hun ) - Animal and Plant Quarantine Agency

Abstract


Background: Canine distemper virus (CDV) infection results in high morbidity and mortality in dogs. There has been no report about isolation of Korean CDV since 1980 in Korea.

Objectives: To investigate the biological properties and the genetic characterization of Korean CDV.

Methods: Vero cells expressing dog signaling lymphocyte activation molecule (dSLAM) gene named as Vero/dSLAM were used to isolate CDV using 17 samples. Diagnostic methods such as cytopathic effects, immunofluorescence assay, peroxidase linked assay, electron microscopy, rapid immunodiagnostic assay, and reverse transcription polymerase chain reaction were used to confirm the Korean CDV isolate as a CDV. The genetic analysis was performed through cloning and sequencing of hemagglutinin gene of CDV isolate.

Results: A virus propagated in Vero/dSLAM cell was confirmed as CDV (CD1901 strain) based on the above methods. The CD1901 strain showed the highest viral titer (105.5 50% tissue culture infectious dose [TCID50]/mL) in the Vero/dSLAM cells at 4 days post inoculation, but did not form a fork on chorioallantoic membrane of 7-day-old egg. Ribavirin, a nucleotide analogue anti-viral agent, inhibits moderately the Korean CDV propagation in the Vero/dSLAM cells. The nucleotide and amino acid sequences of the H gene of CD1901 strain were compared with those of other CDV strains. The CD1901 strain belonged to Asia 1 group and had the highest similarity (99.9%) with the BA134 strain, which was isolated in China in 2008.

Conclusions: We constructed successfully Vero/dSLAM and isolated one Korean CDV isolate (CD1901 strain) from a naturally infected dog. The CD1901 strain belonged to Asia 1 genotype.

키워드

Canine distemper; virus isolation; SLAM

원문 및 링크아웃 정보

  

등재저널 정보