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The changes of immune-related molecules within the ileal mucosa of piglets infected with porcine circovirus type 2

Journal of Veterinary Science 2020년 21권 5호 p.78 ~ 78
Shi Fengyang, Li Qiuming, Zou Zhanming, Wang Yang, Hou Xiaolin, Zhang Yonghong, Song Qinye, Zhou Shuanghai, Li Huanrong,
소속 상세정보
 ( Shi Fengyang ) - Beijing University of Agriculture College of Animal Science and Technology
 ( Li Qiuming ) - Beijing University of Agriculture College of Animal Science and Technology
 ( Zou Zhanming ) - Beijing University of Agriculture College of Animal Science and Technology
 ( Wang Yang ) - Beijing University of Agriculture College of Animal Science and Technology
 ( Hou Xiaolin ) - Beijing University of Agriculture College of Animal Science and Technology
 ( Zhang Yonghong ) - Beijing University of Agriculture College of Animal Science and Technology
 ( Song Qinye ) - Hebei Agricultural University College of Animal Science and Technology
 ( Zhou Shuanghai ) - Beijing University of Agriculture College of Animal Science and Technology
 ( Li Huanrong ) - Beijing University of Agriculture College of Animal Science and Technology

Abstract


Background: Enteritis is one of the most frequently reported symptoms in piglets infected with porcine circovirus type 2 (PCV2), but the immunopathogenesis has not been reported.

Objectives: This study examined the effect of a PCV2 infection on the intestinal mucosal immune function through morphological observations and immune-related molecular detection.

Methods: Morphological changes within the ileum of piglets during a PCV2 infection were observed. The expression of the related-molecules was analyzed using a gene chip. The immunocyte subsets were analyzed by flow cytometry. The secretory immunoglobulin A (SIgA) content was analyzed by enzyme-linked immunosorbent assay.

Results: The PCV2 infection caused ileal villus damage, intestinal epithelial cells exfoliation, and an increase in lymphocytes in the lamina propria at 21 days post-infection. Differentially expressed genes occurred in the defense response, inflammatory response, and the complement and coagulation cascade reactions. Most of them were downregulated significantly at the induction site and upregulated at the effector site. The genes associated with SIgA production were downregulated significantly at the induction site. In contrast, the expression of the Toll-like receptor-related genes was upregulated significantly at the effector site. The frequencies of dendritic cells, B cells, and CD8+T cells were upregulated at the 2 sites. The SIgA content decreased significantly in the ileal mucosa.

Conclusions: PCV2 infections can cause damage to the ileum that is associated with changes in immune-related gene expression, immune-related cell subsets, and SIgA production. These findings elucidated the molecular changes in the ileum after a PCV2 infection from the perspective of intestinal mucosal immunity, which provides insights into a further study for PCV2-induced enteritis.

키워드

PCV2; immunity; ileum; inflammation; gene chip

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