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Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro

Journal of Veterinary Science 2020년 21권 5호 p.80 ~ 80
Liang Xiuli, Zhang Xiaojun, Lian Kaiqi, Tian Xiuhua, Zhang Mingliang, Wang Shiqiong, Chen Cheng, Nie Cunxi, Pan Yun, Han Fangfang, Wei Zhanyong, Zhang Wenju,
소속 상세정보
 ( Liang Xiuli ) - Shihezi University College of Animal Science and Technology
 ( Zhang Xiaojun ) - Anyang Institute of Technology
 ( Lian Kaiqi ) - Anyang Institute of Technology
 ( Tian Xiuhua ) - Anyang County Agricultural and Rural Bureau
 ( Zhang Mingliang ) - Anyang Institute of Technology
 ( Wang Shiqiong ) - Henan Agricultural University College of Animal Husbandry
 ( Chen Cheng ) - Shihezi University College of Animal Science and Technology
 ( Nie Cunxi ) - Shihezi University College of Animal Science and Technology
 ( Pan Yun ) - Henan Yihongshancheng Bio-Tech Co. Ltd.
 ( Han Fangfang ) - Henan Agricultural University College of Animal Husbandry
 ( Wei Zhanyong ) - Henan Agricultural University College of Animal Husbandry
 ( Zhang Wenju ) - Shihezi University College of Animal Science and Technology

Abstract


Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions.

Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments.

Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID50). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development.

Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 μg/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log10TCID50 of 62.5 μg/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 μg/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups.

Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.

키워드

Transmissible gastroenteritis virus; infections; viruses; RNA messenger

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