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Differential Expressions of Aquaporin Subtypes in Female Reproductive Tract of Mice

발생과생식 2020년 24권 3호 p.177 ~ 185
임지우, 이채영, 김동환, 배혜란,
소속 상세정보
임지우 ( Im Ji-Woo ) - Dong-A University College of Medicine Department of Physiology
이채영 ( Lee Chae-Young ) - Dong-A University College of Medicine Department of Physiology
김동환 ( Kim Dong-Hwan ) - Dong-A University Human Life Research Center
배혜란 ( Bae Hae-Rahn ) - Dong-A University College of Medicine Department of Physiology

Abstract


Although many aquaporin (AQP) transcripts have been demonstrated to express in the female reproductive tract, the defined localizations and functions of AQP subtype proteins remain unclear. In this study, we investigated the expression of AQP1, AQP3, AQP5, AQP6, and AQP9 proteins in female reproductive tract of mouse and characterized their precise localizations at the cellular and subcellular levels. Immunofluorescence analyses for AQP1, AQP3, AQP6, and AQP9 showed that these proteins were abundantly expressed in female reproductive tract and that intense immunoreactivities were observed in mucosa epithelial cells with a subtype-specific pattern. The most abundant aquaporin in both vagina and uterine cervix was AQP3. Each of AQP1, AQP3, AQP6, and AQP9 exhibited its distinct distribution in stratified squamous or columnar epithelial cells. AQP9 expression was predominant in oviduct and ovary. AQP1, AQP3, AQP6, and AQP9 proteins were mostly seen in apical membrane of ciliated epithelial cells of the oviduct as well as in both granulosa and theca cells of ovarian follicles. Most of AQP subtypes were also expressed in surface epithelial cells and glandular cells of endometrium in the uterus, but their expression levels were relatively lower than those observed in the vagina, uterine cervix, oviduct and ovary.
This is the first study to investigate the expression and localization of 5 AQP subtype proteins simultaneously in female reproductive tract of mouse. Our results suggest that AQP subtypes work together to transport water and glycerol efficiently across the mucosa epithelia for lubrication, proliferation, energy metabolism and pH regulation in female reproductive tract.

키워드

Aquaporin 1 (AQP1); AQP3; AQP5; AQP6; AQP9; Female reproductive tract; Immunofluorescence

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