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경골 파혈산동탕(破血散疼湯)이 골절 생쥐의 골 유합에 미치는 영향

Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice

한방재활의학과학회지 2020년 30권 4호 p.1 ~ 16
신우석, Parichuk Kira, 차윤엽,
소속 상세정보
신우석 ( Shin Woo-Suk ) - Sangji University Graduate School
 ( Parichuk Kira ) - Sangji University College of Korean Medicine Department of Pharmacology
차윤엽 ( Cha Yun-Yeop ) - Sangji University Korean Medicine Hospital Department of Korean Rehabilitation Medicine

Abstract


Objectives: The main purpose of this study was to evaluate the bone healing effect of Pahyeolsandong-tang (PHT)(Poxiesanteng-tang) extract in tibia fracture-induced mice.

Methods: PHT was extracted using a solution of 35% ethanol in 60°C for 8 hours. Mice were randomly divided into 4 groups (normal, control, PHT 50 and PHT 100). Mice of experimental groups were medicated with PHT 50 or 100 mg/kg for 7 to 21 days. To clarify the effect of bone fracture healing, relative messenger RNA (mRNA) expressions of osteocalcin (OCN), runt-related transcription factor 2 (Runx2), osterix (OSX), Sox9, collagen type Ⅱ alpha 1 chain (Col2a1), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) were examined.

Results: In in vitro experiment, relative mRNA expression of OCN, Runx2, Col2a1 was significantly increased in PHT treated group to compare with control differentiation group. In in vivo experiment, relative mRNA expression of OCN, Runx2, OSX, Sox9, Col2a1, RANKL, OPG was significantly increased in PHT treated group.

Conclusions: This study showed that PHT accelerates bone fracture healing through the activation of osteoclasts and osteoblasts. It was showed that PHT significantly promotes osteoblasts differentiation by osteoblast differentiation markers such as OCN, Runx2, Col1a2. Also it was investigated that PHT had stimulatory effect on osteoblasts function through enhancing OCN, Runx2, OSX, Sox9, Col2a1 and, osteoclasts function through enhancing RANKL and OPG markers. PHT effectively promotes bone fracture healing process through activation of osteoblasts and osteoclasts

키워드

Pahyeolsandong-tang (Poxiesanteng-tang); Osteoblasts; Osteoclasts; Chondrogenesis; Fracture healing

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