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육미지황탕(六味地黃湯)이 대퇴골절 동물모델의 골절 유합인자 및 형태학적 변화에 미치는 영향

Healing Effect of Yukmijihwang-tang on Fracture Factor and Morphological Changes in Femur Fractured Mice

한방재활의학과학회지 2020년 30권 4호 p.17 ~ 30
김현석, 전동휘, 오민석,
소속 상세정보
김현석 ( Kim Hyun-Seok ) - Daejeon University College of Korean Medicine Department of Korean Medicine Rehabilitation
전동휘 ( Jeon Dong-Hwi ) - Daejeon University College of Korean Medicine Department of Korean Medicine Rehabilitation
오민석 ( Oh Min-Seok ) - Daejeon University College of Korean Medicine Department of Korean Medicine Rehabilitation

Abstract


Objectives: The purpose of this study is to evaluate the healing effect of Yukmijihwang- tang (YM) on femur fractured mice.

Methods: Mice were randomly divided into 6 groups: normal, control, positive control, YM with low, medium, high dosage each. All groups were prepared with femur fracture and treated diffrently. In order to measure bone regeneration effects, we analysed the levels of cyclooxygenase-2 (COX2), bone morphogenetic protein-2 (BMP2), collagen type Ⅱ alpha 1 chain (Col2a1), Sox9, runt-related transcription factor 2 (Runx2), and osterix genes expressed in bone. For morphological analysis, muscles were removed and femur was observed with naked eye.

Results: COX2 gene expression in bone marrow significantly decreased. BMP2 gene expression significantly increased. Col2a1 gene expression significantly increased. Sox9 gene expression increased as well. Runx2 gene expression in bone marrow increased, but there was no statistical significance. Osterix gene expression significantly increased. Union of the fracture site progressed more in YM group compared to the control group. The fracture union score was significantly decreased in YM group compared to the control group.

Conclusions: YM showed anti-inflammatory effect, promoted bone regeneration by stimulating the bone regeneration factor. In conclusion, YM can help fracture healing and it well be applied clinically to patients with fracture.

키워드

Korean traditional medicine; Anti-inflammatory agents; Gene expression; Bone regeneration; Fracture healing

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