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Neuroprotective effect of Allium hookeri against H2O2-induced PC12 cell cytotoxicity by reducing oxidative stress

Food Science and Biotechnology 2020년 29권 11호 p.1519 ~ 1530
노상호, 유소현, 김건희, 박현진,
소속 상세정보
노상호 ( Rho Sang-Ho ) - Korea University College of Life Science and Biotechnology Department of Biotechnology
유소현 ( You So-Hyeon ) - Duksung Women’s University Department of Health Functional Materials
김건희 ( Kim Gun-Hee ) - Duksung Women’s University Department of Health Functional Materials
박현진 ( Park Hyun-Jin ) - Korea University College of Life Science and Biotechnology Department of Biotechnology

Abstract


In this study, the anti-oxidative and neuro-protective effects of ethanolic extracts of the dried roots of Allium hookeri were investigated. Total phenolic contents and total flavonoid contents of A. hookeri extract depended on the ethanol concentrations used (50, 70 and 95%). In order to evaluate radical scavenging activity, DPPH and ABTS radical scavenging assays and ferric reducing powers were evaluated. The results showed the 95% ethanol extract of A. hookeri (95AH) had higher phenolic and flavonoid contents, and greater radical scavenging activities than 50 or 70% ethanol extracts of A. hookeri. The neuro-protective effects of 95AH were evaluated using H2O2-treated PC12 neuronal cells. Treatment of 95AH increased cell viability and superoxide dismutase and glutathione peroxidase activities, reduced lactate dehydrogenate release, reduced reactive oxygen species production, and increased Bcl-2/Bax ratio. HPLC revealed 95AH was rich in phenolics, especially catechin. These results demonstrate 95AH has substantial anti-oxidative and neuro-protective effects against H2O2-induced oxidative stress.

키워드

Allium hookeri; Antioxidant; Neuro-protection; Phenolics; Flavonoids

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