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Comparison of gamma irradiation and heating treatment on cytotoxicity, insulinotropic activity, and molecular structure change of mistletoe viscothionin

Food Science and Biotechnology 2020년 29권 11호 p.1531 ~ 1539
박종흠, 김재경, 송범석,
소속 상세정보
박종흠 ( Park Jong-Heum ) - Korea Atomic Energy Research Institute Advanced Radiation Technology Institute
김재경 ( Kim Jae-Kyung ) - Korea Atomic Energy Research Institute Advanced Radiation Technology Institute
송범석 ( Song Beom-Seok ) - Korea Atomic Energy Research Institute Advanced Radiation Technology Institute

Abstract


Mistletoe offers health-promoting effects; however, it has toxicity, requiring careful application. Viscothionin is a polypeptide of mistletoe that while contributing to toxicity also demonstrates anti-cancer and anti-diabetic activities. The aim of this study was to evaluate whether gamma irradiation or heating treatment could selectively reduce viscothionin-mediated cytotoxicity. Gamma irradiation effectively inhibited viscothionin-induced cytotoxicity to RIN5mF cells, but heating treatment did not affect its cytotoxicity. Both heating and gamma irradiation further increased the insulinotropic activity of viscothionin, whereas the effect of gamma irradiation was dose-dependent and diminished above 20 kGy. Structural analysis showed that gamma irradiation significantly altered the ordered structure of viscothionin, unlike heating treatment, resulting in a change of its molecular properties, which could be linked to the observed changes in the cytotoxicity and insulinotropic activity of the polypeptide. These results suggest gamma irradiation as an alternative method for minimizing viscothionin toxicity without interfering with anti-diabetic effect.

키워드

Polypeptide of mistletoe; Toxicity; Gamma irradiation; Heating treatment; Structural analysis

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