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Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes

Food Science and Biotechnology 2020년 29권 11호 p.1541 ~ 1551
김석진, 최수임, 장미란, Jeong Yu-Lah, 강창호, 김건희,
소속 상세정보
김석진 ( Kim Suk-Jin ) - Duksung Women’s University Department of Bio-Health Convergence Major
최수임 ( Choi Soo-Im ) - Duksung Women’s University Department of Bio-Health Convergence Major
장미란 ( Jang Mi-Ran ) - Purdue University Department of Food Science
 ( Jeong Yu-Lah ) - MEDIOGEN Co. Ltd.
강창호 ( Kang Chang-Ho ) - MEDIOGEN Co. Ltd.
김건희 ( Kim Gun-Hee ) - Duksung Women’s University Department of Bio-Health Convergence Major

Abstract


This study evaluated the anti-adipogenic effects and mechanisms underlying the action of Lactobacillus fermentum MG4231 and MG4244 strains on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes. Treatment with cell-free extracts (CFEs) from the two strains reduced lipid accumulation and intracellular triglyceride production in 3T3-L1 adipocytes by more than 50%. The inhibitory effects of L. fermentum on lipid accumulation were mediated by the downregulation of FAS and aP2 resulting from the inhibition of PPARγ and C/EBPα gene expression. Moreover, AMPK and HSL phosphorylation was upregulated by CFE treatment. These results indicated that the anti-adipogenic and lipolysis activities of L. fermentum strains were caused by increased AMPK and HSL phosphorylation. Both strains displayed high leucine arylamidase and β-galactosidase enzymatic activity, with excellent adhesion to epithelial cells. Therefore, we identified L. fermentum as potential new probiotics for the prevention of obesity.

키워드

L. fermentum; MG4231; MG4244; Adipogenesis; Probiotics

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