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Simultaneous analysis of thirteen phytohormones in fruits and vegetables by SPE-HPLC?DAD

Food Science and Biotechnology 2020년 29권 11호 p.1587 ~ 1595
Zhu Song, Chen Shang-Wei, Li Yue,
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 ( Zhu Song ) - Jiangnan University State Key Laboratory of Food Science and Technology
 ( Chen Shang-Wei ) - Jiangnan University State Key Laboratory of Food Science and Technology
 ( Li Yue ) - Jiangnan University State Key Laboratory of Food Science and Technology

Abstract


Determination of phytohormones have attracted increasing attentions in food safety field. In this study, an efficient and quantitative method was developed which can simultaneously determinate thirteen phytohormones in fruits and vegetables using solid phase extraction (SPE) combined with high performance liquid chromatography?diode array detection (HPLC?DAD). The samples were extracted with 80% methanol containing 0.5% (V/V) formic acid, and the extracts were then concentrated and purified using primary secondary amine (PSA) and C18 tandem dual SPE cartridges. The analytes were separated on a Waters XBridge™ C18 column and eluated utilizing a gradient elution program of water and methanol. Mean recoveries of the thirteen analytes varied from 74.69 to 92.40%, with relative standard deviations?

키워드

Phytohormone; High performance liquid chromatography?diode array detection; Simultaneous analysis; Fruit and vegetable

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