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Anti-Biofilm Effect of Egg Yolk Phosvitin by Inhibition of Biomass Production and Adherence Activity against Streptococcus mutans

한국축산식품학회지 2020년 40권 6호 p.1001 ~ 1013
김현중, 이재훈, 안동욱, 백현동,
소속 상세정보
김현중 ( Kim Hyeon-Joong ) - Konkuk University Department of Food Science and Biotechnology of Animal Resources
이재훈 ( Lee Jae-Hoon ) - Konkuk University Department of Food science and Biotechnology of Animal Resources
안동욱 ( Ahn Dong-Uk ) - Iowa State University Department of Animal Science
백현동 ( Paik Hyun-Dong ) - Konkuk University Department of Food Science and Biotechnology of Animal Resources

Abstract


The formation of biofilms on the enamel surface of teeth by Streptococcus mutans is an important step in dental plaque formation, demineralization, and early caries because the biofilm is where other bacteria involved in dental caries attach, grow, and proliferate. The objectives of this study were to determine the effect of phosvitin (PSV) on the biofilm formation, exopolysaccharides (EPS) production, adherence activity of S. mutans, and the expression of genes related to the compounds essential for biofilm formation (quorum-sensing inducers and components of biofilm matrix) by S. mutans. PSV significantly reduced the biofilm-forming activity of S. mutans and increased the degradation of preformed biofilms by S. mutans. PSV inhibited the adherence activity of S. mutans by 31.9%?33.6%, and the production of EPS by 62%?65% depending upon the strains and the amount of PSV added. The expressions of genes regulating the production of EPS and the quorum-sensing-inducers (gtfA, gtfD, ftf, relA, vicR, brpA, and comDE) in all S. mutans strains were down-regulated by PSV, but gtfB was down-regulated only in S. mutans KCTC 5316. Therefore, the anti-biofilm-forming activity of PSV was accomplished through the inhibition of biofilm formation, adherence activity, and the production of quorum-sensing inducers and EPS by S. mutans.

키워드

phosvitin; Streptococcus mutans; biofilm formation

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